Glutathione metabolism by γ-glutamyltranspeptidase leads to lipid peroxidation: Characterization of the system and relevance to hepatocarcinogenesis

Avishay Abraham Stark, Errol Zeiger, Dennis A. Pagano

Research output: Contribution to journalArticlepeer-review

Abstract

Glutathione (GSH)-driven lipid peroxidation (LPO) in vitro was catalyzed by γ-glutamyltranspeptidase (GGT; EC 2.3.2.2.). The reaction required iron, iron chelators and oxygen, was accelerated by glycylglycine (gly)2, a GGT enhancer, and was inhibited by the GGT inhibitors serine-borate and acivicin. LPO occurred at rat plasma concentrations of GSH and transferrin, and in the presence of putative physiological chelators such as citrate and ADP. GSH-driven LPO was inhibited by butylated hydroxytoluene, but not by catalase, peroxidase or superoxide dismutase. These results suggest that metabolism of GSH initiated by GGT may lead to oxidative damage. Such oxidative damage may be induced in vivo by GSH in proximity to GGT-rich preneoplastic foci in rat liver.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalCarcinogenesis
Volume14
Issue number2
DOIs
StatePublished - Feb 1993

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