Glutathione II. Chemical aspects of azoester procedure for oxidation to disulfide

Nechama S. Kosower, Keum Ryul Song, Edward M. Kosower, Walter Correa

Research output: Contribution to journalArticlepeer-review

Abstract

1. 1. The reaction of methyl phenyldiazenecarboxylate ("azoester", C6H6H5N = NCOOCH3) with GSH produces methyl phenyldiazanecarboxylate ("hydrazoester", C6H5NHNHCOOCH3) and GSSG. The stoichiometry of the reaction follows the equation: 2 GSH + C6H5N = NCOOCH3 → C6H5NHNHCOOCH3 + GSSG 2. 2. The rate of the reaction depends upon the concentration of azoester and GSH. 3. 3. Evidence for an intermediate composed of GSSG and hydrazoester is presented. 4. 4. The mechanism of the overall reaction between azoester and GSH involves two intermediates. 5. 5. Other reactions of azoester of importance to the GSH oxidation procedure are described.

Original languageEnglish
Pages (from-to)8-14
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume192
Issue number1
DOIs
StatePublished - 7 Oct 1969
Externally publishedYes

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