TY - JOUR
T1 - Glutathione I. The methyl phenyldiazenecarboxylate (azoester) procedure for intracellular oxidation
AU - Kosower, Nechama S.
AU - Song, Keum Ryul
AU - Kosower, Edward M.
N1 - Funding Information:
The authors are grateful to the National Institutes of Health, the Army Research Office (Durham) and the National Science Foundation for support. N.S.K. is a Career Development Awardee (I-K4-GM-38 889) of the National Institute of General Medical Sciences, U.S. Public Health Service. M.J.E. and D.M.C.T. are participants in summer research program for undergraduate students
PY - 1969/10/7
Y1 - 1969/10/7
N2 - 1. 1. An effective new reagent, methyl phenyldiazenecarboxylate ("azoester", C6H5N = NCOOCH3) for the intracellular oxidation of GSH to GSSG is described. 2. 2. Using human erythrocytes as the test system, the reaction of azoester with GSH is shown to be rapid (complete within 1-2 min under the usual conditions), independent of O2, approximately stoichiometric and effective even at 1°. 3. 3. The rate and extent of GSH regeneration within the cell can easily be followed after intracellular oxidation with azoester. 4. 4. Azoester is a convenient reagent available for the intracellular oxidation of GSH to GSSG under conditions in which regeneration of GSH is required.
AB - 1. 1. An effective new reagent, methyl phenyldiazenecarboxylate ("azoester", C6H5N = NCOOCH3) for the intracellular oxidation of GSH to GSSG is described. 2. 2. Using human erythrocytes as the test system, the reaction of azoester with GSH is shown to be rapid (complete within 1-2 min under the usual conditions), independent of O2, approximately stoichiometric and effective even at 1°. 3. 3. The rate and extent of GSH regeneration within the cell can easily be followed after intracellular oxidation with azoester. 4. 4. Azoester is a convenient reagent available for the intracellular oxidation of GSH to GSSG under conditions in which regeneration of GSH is required.
UR - http://www.scopus.com/inward/record.url?scp=0014667271&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(69)90002-6
DO - 10.1016/0304-4165(69)90002-6
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AN - SCOPUS:0014667271
SN - 0304-4165
VL - 192
SP - 1
EP - 7
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 1
ER -