TY - JOUR
T1 - Glucose transport activity and ligand binding (cytochalasin B, IAPS-forskolin) of chimeric constructs of GLUT2 and GLUT4 expressed in COS-7-cells
AU - Wandel, Sonja
AU - Buchs, Andreas
AU - Schürmann, Annette
AU - Summers, Scott A.
AU - Powers, Alvin C.
AU - Shanahan, Michael F.
AU - Joost, Hans Georg
PY - 1996/10/2
Y1 - 1996/10/2
N2 - Chimeric constructs of glucose transporters GLUT2 and GLUT4 were transiently expressed in COS-7 cells in order to determine regions of the proteins responsible for their differences in activity and ligand binding. Exchange of the C-terminal tail (aa 479-509) of GLUT4 failed to affect glucose transport activity assayed at 1 mM glucose or ligand binding (cytochalasin B, IAPS-forskolin). In contrast, exchange of the C-terminal half of GLUT4 (aa 222-509) for that of GLUT2 markedly reduced ligand binding (K(d)) of cytochalasin B binding 1.88 ± 0.2 μM vs. 0.21 ± 0.06 in the wild-type GLUT4), and moderately (25%) reduced glucose transport activity. These data support the conclusion that the domains determining differences in Ligand binding between GLUT4 and GLUT2 are located in the C-terminal half of the glucose transporters.
AB - Chimeric constructs of glucose transporters GLUT2 and GLUT4 were transiently expressed in COS-7 cells in order to determine regions of the proteins responsible for their differences in activity and ligand binding. Exchange of the C-terminal tail (aa 479-509) of GLUT4 failed to affect glucose transport activity assayed at 1 mM glucose or ligand binding (cytochalasin B, IAPS-forskolin). In contrast, exchange of the C-terminal half of GLUT4 (aa 222-509) for that of GLUT2 markedly reduced ligand binding (K(d)) of cytochalasin B binding 1.88 ± 0.2 μM vs. 0.21 ± 0.06 in the wild-type GLUT4), and moderately (25%) reduced glucose transport activity. These data support the conclusion that the domains determining differences in Ligand binding between GLUT4 and GLUT2 are located in the C-terminal half of the glucose transporters.
KW - 3-[I]Ioda-4-azidophenetylamido-7-O-succinyldeacetyl-forskolin
KW - Cytochalasin B
KW - Glucose transport
KW - Insulin-regulated glucose transporter GLUT4
UR - http://www.scopus.com/inward/record.url?scp=0007516840&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(96)00111-3
DO - 10.1016/0005-2736(96)00111-3
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C2 - 8865815
AN - SCOPUS:0007516840
SN - 0005-2736
VL - 1284
SP - 56
EP - 62
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 1
ER -