@article{cdd5202d33e24044bcafee4de521c85f,
title = "Glial ER and GAP junction mediated Ca2+ waves are crucial to maintain normal brain excitability",
abstract = "Astrocytes play key roles in regulating multiple aspects of neuronal function from invertebrates to humans and display Ca2+ fluctuations that are heterogeneously distributed throughout different cellular microdomains. Changes in Ca2+ dynamics represent a key mechanism for how astrocytes modulate neuronal activity. An unresolved issue is the origin and contribution of specific glial Ca2+ signaling components at distinct astrocytic domains to neuronal physiology and brain function. The Drosophila model system offers a simple nervous system that is highly amenable to cell-specific genetic manipulations to characterize the role of glial Ca2+ signaling. Here we identify a role for ER store-operated Ca2+ entry (SOCE) pathway in perineurial glia (PG), a glial population that contributes to the Drosophila blood–brain barrier. We show that PG cells display diverse Ca2+ activity that varies based on their locale within the brain. Ca2+ signaling in PG cells does not require extracellular Ca2+ and is blocked by inhibition of SOCE, Ryanodine receptors, or gap junctions. Disruption of these components triggers stimuli-induced seizure-like episodes. These findings indicate that Ca2+ release from internal stores and its propagation between neighboring glial cells via gap junctions are essential for maintaining normal nervous system function.",
keywords = "Ca waves, Drosophila, perineurial glia, seizures, store-operated Ca entry",
author = "Shirley Weiss and Clamon, {Lauren C.} and Manoim, {Julia E.} and Ormerod, {Kiel G.} and Moshe Parnas and Littleton, {J. Troy}",
note = "Publisher Copyright: {\textcopyright} 2021 Wiley Periodicals LLC.",
year = "2022",
month = jan,
doi = "10.1002/glia.24092",
language = "אנגלית",
volume = "70",
pages = "123--144",
journal = "GLIA",
issn = "0894-1491",
publisher = "John Wiley & Sons Inc.",
number = "1",
}