TY - JOUR
T1 - Gibberellins accumulate in the elongating endodermal cells of Arabidopsis root
AU - Shani, Eilon
AU - Weinstain, Roy
AU - Zhang, Yi
AU - Castillejo, Cristina
AU - Kaiserli, Eirini
AU - Chory, Joanne
AU - Tsien, Roger Y.
AU - Estelle, Mark
PY - 2013/3/19
Y1 - 2013/3/19
N2 - Plant hormones are small-molecule signaling compounds that are collectively involved in all aspects of plant growth and development. Unlike animals, plants actively regulate the spatial distribution of several of their hormones. For example, auxin transport results in the formation of auxin maxima that have a key role in developmental patterning. However, the spatial distribution of the other plant hormones, including gibberellic acid (GA), is largely unknown. To address this, we generated two bioactive fluorescent GA compounds and studied their distribution in Arabidopsis thaliana roots. The labeled GAs specifically accumulated in the endodermal cells of the root elongation zone. Pharmacological studies, along with examination of mutants affected in endodermal specification, indicate that GA accumulation is an active and highly regulated process. Our results strongly suggest the presence of an active GA transport mechanism that would represent an additional level of GA regulation.
AB - Plant hormones are small-molecule signaling compounds that are collectively involved in all aspects of plant growth and development. Unlike animals, plants actively regulate the spatial distribution of several of their hormones. For example, auxin transport results in the formation of auxin maxima that have a key role in developmental patterning. However, the spatial distribution of the other plant hormones, including gibberellic acid (GA), is largely unknown. To address this, we generated two bioactive fluorescent GA compounds and studied their distribution in Arabidopsis thaliana roots. The labeled GAs specifically accumulated in the endodermal cells of the root elongation zone. Pharmacological studies, along with examination of mutants affected in endodermal specification, indicate that GA accumulation is an active and highly regulated process. Our results strongly suggest the presence of an active GA transport mechanism that would represent an additional level of GA regulation.
KW - Ethylene
KW - Fluorescent labeling
KW - Hormone labeling
KW - Root development
KW - Root growth
UR - http://www.scopus.com/inward/record.url?scp=84875242346&partnerID=8YFLogxK
U2 - 10.1073/pnas.1300436110
DO - 10.1073/pnas.1300436110
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AN - SCOPUS:84875242346
SN - 0027-8424
VL - 110
SP - 4834
EP - 4839
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12
ER -