Gibberellin and abscisic acid transporters facilitate endodermal suberin formation in Arabidopsis

Jenia Binenbaum, Nikolai Wulff, Lucie Camut, Kristian Kiradjiev, Moran Anfang, Iris Tal, Himabindu Vasuki, Yuqin Zhang, Lali Sakvarelidze-Achard, Jean Michel Davière, Dagmar Ripper, Esther Carrera, Ekaterina Manasherova, Shir Ben Yaakov, Shani Lazary, Chengyao Hua, Vlastimil Novak, Christoph Crocoll, Roy Weinstain, Hagai CohenLaura Ragni, Asaph Aharoni, Leah R. Band*, Patrick Achard*, Hussam Hassan Nour-Eldin*, Eilon Shani*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The plant hormone gibberellin (GA) regulates multiple developmental processes. It accumulates in the root elongating endodermis, but how it moves into this cell file and the significance of this accumulation are unclear. Here we identify three NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER (NPF) transporters required for GA and abscisic acid (ABA) translocation. We demonstrate that NPF2.14 is a subcellular GA/ABA transporter, presumably the first to be identified in plants, facilitating GA and ABA accumulation in the root endodermis to regulate suberization. Further, NPF2.12 and NPF2.13, closely related proteins, are plasma membrane-localized GA and ABA importers that facilitate shoot-to-root GA12 translocation, regulating endodermal hormone accumulation. This work reveals that GA is required for root suberization and that GA and ABA can act non-antagonistically. We demonstrate how the clade of transporters mediates hormone flow with cell-file-specific vacuolar storage at the phloem unloading zone, and slow release of hormone to induce suberin formation in the maturation zone.

Original languageEnglish
Pages (from-to)785-802
Number of pages18
JournalNature Plants
Volume9
Issue number5
DOIs
StatePublished - May 2023

Funding

FundersFunder number
Rothamsted Research
European Research Council757683-RobustHormoneTrans
Human Frontier Science ProgramHFSP—RGY0075/2015
Danmarks GrundforskningsfondDNRF99
Israel Science Foundation3419/20, 2378/19
Ministère de l'Education Nationale, de l'Enseignement Superieur et de la Recherche
Centre National de la Recherche Scientifique

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