Genotype phenotype correlations in Israeli colorectal cancer patients

Sigal Starinsky, Arie Figer, Edna Ben-Asher, Ravit Geva, Dov Flex, Herma H. Fidder, Jamal Zidan, Doron Lancet, Eitan Friedman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

While genetic factors clearly play a key role in colorectal cancer (CRC) pathogenesis and in determining its phenotypic features, the precise genes that involved are largely unknown. To gain insight into these genes, consecutive Israeli CRC patients were genotyped using SNPs from within candidate genes: APC, β-Catenin, K-RAS, DCC, P16, PTEN, RB1, P15, APOE, ERCC2, P53, MTHFR and hMSH2. Genotyping of consecutive, unselected colorectal cancer patients was done mostly by utilizing the MassARRAY technology (Sequenom) and to a lesser extent DGGE, ARMS and direct DNA sequencing. Correlation of genotypes with specific phenotypic features was carried out for all patients and separately for the Ashkenazim. Overall, 456 patients were analyzed, the majority (64.25%) being of Ashkenazi origin; mean age at diagnosis was 65.6 ± 14 (range 25-90 years), and the mean follow-up was 4.7 ± 0.28 (range 0-30 years). Statistically significant associations were noted between SNPs in β-catenin and APOE and a positive family history of cancer (β-catenin: p=0.034, APOE: p=0.033); tumor location and a DCC SNP (p=0.038) and the P53 R72P mutation and survival (p=0.0336). In Ashkenazi patients, ERCC2 and MTHFR genes' SNPs were associated with age at diagnosis (ERCC2: p=0.025, MTHFR: p=0.0005); a P53 polymorphism, APOE and Rb SNPs with a family history of cancer (P53 p=0.034; APOE p=0.04, Rb p=0.022); DCC SNP with tumor location (p=0.014); and p15 SNP with tumor grade (p=0.032). This preliminary study shows that genetic factors play a role in determining CRC phenotypic features and that a larger cohort with longer follow-up is clearly needed.

Original languageEnglish
Pages (from-to)58-73
Number of pages16
JournalInternational Journal of Cancer
Volume114
Issue number1
DOIs
StatePublished - 10 Mar 2005
Externally publishedYes

Keywords

  • Colorectal cancer
  • Genotype-phenotype correlation
  • Hardy Weinberg equilibrium
  • SNP
  • Tumor phenotype

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