Genome-wide identification and phenotypic characterization of seizure-associated copy number variations in 741,075 individuals

Epi25 Collaborative, Columbia University Institute for Genomic Medicine analysis group, Epi25 sequencing, analysis, project management, and browser development at the Broad Institute, Epi25 executive committee, Epi25 strategy, phenotyping, analysis, informatics, and project management committees, Authors from individual Epi25 cohorts:

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Copy number variants (CNV) are established risk factors for neurodevelopmental disorders with seizures or epilepsy. With the hypothesis that seizure disorders share genetic risk factors, we pooled CNV data from 10,590 individuals with seizure disorders, 16,109 individuals with clinically validated epilepsy, and 492,324 population controls and identified 25 genome-wide significant loci, 22 of which are novel for seizure disorders, such as deletions at 1p36.33, 1q44, 2p21-p16.3, 3q29, 8p23.3-p23.2, 9p24.3, 10q26.3, 15q11.2, 15q12-q13.1, 16p12.2, 17q21.31, duplications at 2q13, 9q34.3, 16p13.3, 17q12, 19p13.3, 20q13.33, and reciprocal CNVs at 16p11.2, and 22q11.21. Using genetic data from additional 248,751 individuals with 23 neuropsychiatric phenotypes, we explored the pleiotropy of these 25 loci. Finally, in a subset of individuals with epilepsy and detailed clinical data available, we performed phenome-wide association analyses between individual CNVs and clinical annotations categorized through the Human Phenotype Ontology (HPO). For six CNVs, we identified 19 significant associations with specific HPO terms and generated, for all CNVs, phenotype signatures across 17 clinical categories relevant for epileptologists. This is the most comprehensive investigation of CNVs in epilepsy and related seizure disorders, with potential implications for clinical practice.

Original languageEnglish
Article number4392
JournalNature Communications
Volume14
Issue number1
DOIs
StatePublished - Dec 2023

Funding

FundersFunder number
Epilepsy NeuroGenetics InitiativeT32HG002295
National Science Foundation2017240332
National Science Foundation
National Institutes of HealthUL1TR001878
National Institutes of Health
National Heart, Lung, and Blood Institute
National Human Genome Research Institute
National Institute of Neurological Disorders and StrokeK02 NS112600
National Institute of Neurological Disorders and Stroke
National Center for Advancing Translational Sciences
Children's Hospital of Philadelphia
Hartwell Foundation
University of PennsylvaniaU54 HD086984
University of Pennsylvania
Intellectual and Developmental Disabilities Research Center
Perelman School of Medicine, University of Pennsylvania
Institute for Translational Medicine and Therapeutics
Eunice Kennedy Shriver National Institute of Child Health and Human Development
Wellcome Trust203914/Z/16/Z
Wellcome Trust
Broad Institute5U01HG009088-02, UM1 HG008895
Broad Institute
Deutsche ForschungsgemeinschaftHE5415/5-1, HE5415/6-1, HE5415/3-1, HE5415/7-1
Deutsche Forschungsgemeinschaft

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