Genetically engineered pfabA pfabR bacteria: An electrochemical whole cell biosensor for detection of water toxicity

Tova Neufeld, Dvora Biran, Rachela Popovtzer, Tamir Erez, Eliora Z. Ron, Judith Rishpon

Research output: Contribution to journalArticlepeer-review

Abstract

We describe here a bacterial sensor for electrochemical detection of toxic chemicals. The sensor constitutes recombinant bacteria harboring plasmids encoding the fabA and fabR genes and has high-resolution amperometric response to membrane-damaging chemicals. For example, it can detect phenol at concentrations ranging between 1.6 and 16 ppm within 20 min. The high sensitivity is achieved by using the fabA promoter fused to a reporter gene-encoded β-galactosidase on a low copy number plasmid, under the control of the FabR repressor. The use of electrochemical whole cell sensors enables sensitive, fast, easy to operate, and cost-effective detection of water toxicity threats.

Original languageEnglish
Pages (from-to)4952-4956
Number of pages5
JournalAnalytical Chemistry
Volume78
Issue number14
DOIs
StatePublished - 15 Jul 2006

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