Genetic mutation screen in early non-small-cell lung cancer (NSCLC) specimens

Jair Bar*, Maya Damianovich, Goni Hout Siloni, Erel Dar, Yoram Cohen, Marina Perelman, Alon Ben Nun, David Simansky, Alon Yellin, Damien Urban, Amir Onn

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background Testing for genetic abnormalities in epithelial growth factor receptor (EGFR), anaplastic lymphoma receptor tyrosine kinase (ALK), and potentially additional genes is a critical tool in the care of advanced NSCLC. There is conflicting evidence for the role of such tests in early NSCLC. We report a single-institute Sequenom testing for a wide range of mutations and their clinical correlations in early-resected NSCLC specimens. Materials and Methods Early NSCLC paraffin-embedded, formalin-fixed (FFPE) specimens were collected, DNA extracted, and using Sequenom-based matrix-assisted laser desorption/ionization-time of flight analysis, mutations in 22 oncogenes and tumor suppressor genes were evaluated. Clinical data was collected retrospectively. Results The technique was found to be feasible. Thirty-six of 96 patients (37.5%) had any genetic abnormality identified, and 8 (8.3%) had 2 or more mutations. Kirsten rat sarcoma viral oncogene homolog (KRAS) and EGFR were the most common genes to appear mutated (15.6%); phosphatidylinositol-4,5- bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) was the gene to be found most commonly in tumors with co-mutations. Transversions were found mostly in KRAS gene mutations and to be nonprognostic. No difference in the spectrum of mutations was found between squamous-cell and non-squamous-cell lung cancers. Ever-smokers showed a trend for worse prognosis, with a similar spectrum of mutations. Conclusion Sequenom-based mutation screen is feasible using FFPE samples. More than a third of the patients were found to harbor some genetic abnormality, and 8% were found to have more than a single mutated gene. Wide-range gene screens using large sample depositories are required for further insight into the important genes at play in early NSCLC.

Original languageEnglish
Pages (from-to)159-165
Number of pages7
JournalClinical Lung Cancer
Volume15
Issue number2
DOIs
StatePublished - Mar 2014
Externally publishedYes

Keywords

  • Co-mutation
  • FFPE
  • Sequenom
  • Transitions
  • Transversion

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