@article{a4eabd411c3e4c8aaa791222d9d08b61,
title = "Genetic and immunological factors interact in a mouse model of CNS antiphospholipid syndrome",
abstract = "The antiphospholipid syndrome (APS) includes systemic and central nervous system (CNS) pathology associated with antibodies to a complex of phospholipids and β2-glycoprotein I (β2-GPI). We have recently reported the induction of APS associated with behavioral and cognitive deficits in BALB/c female mice that developed 4-5 months after immunization with β2-GPI. In the present study, we examined the influence of genetic factors on the ability to induce experimental APS with CNS involvement by testing several mouse strains immunized with β2-GPI. Female mice from five strains were immunized once with β2-GPI in complete Freund's adjuvant (CFA) or with CFA alone (controls). Autoantibody levels were examined at 1 and 5 months after immunization. Neurological assessment in a staircase test was performed 4-5 months following the immunization. Induction of APS resulted in elevated levels of antibodies against negatively charged phospholipids and β2-GPI in all five mouse strains. Autoantibody levels were significantly higher in Balb/c, ICR, and C57BL/6 mouse strains compared to AKR and C3H. aPL levels dropped significantly more in the C57BL/6 compared to Balb/c mice over a period of 4 months. Hyperactivity reflected by higher number of stairs climbed in 3 min, was induced by APS in the Balb/c and ICR, mouse strains. Exploratory behavior reflected by more frequent rears, was seen in the APS-Balb/c and AKR mice. Hypoactivity and less exploration were seen in the APS-C57BL/6 and C3H mice. The study supports a link between high levels of aPL and behavioral changes in a mouse APS model. Qualitative differences in behavioral patterns may be due to nervous system as well as immune genetic factors. The minimal effect of APS in C57BL/6 mice may provide a suitable background for the study of transgenes in these mice.",
keywords = "Antibodies, Antiphospholipid syndrome, Behavior, Experimental model",
author = "Aviva Katzav and Yulia Litvinjuk and Pick, {Chaim G.} and Miri Blank and Yehuda Shoenfeld and Pinhas Sirota and Joab Chapman",
note = "Funding Information: This study was performed in partial fulfillment of the requirements for a PhD degree of Aviva Katzav, Sackler Faculty of Medicine, Tel Aviv University, Israel. We thank Dr. Naam Kariv for invaluable advice and help. Supported by the Shreiber Fund for Medical Research.",
year = "2006",
month = may,
day = "15",
doi = "10.1016/j.bbr.2006.01.015",
language = "אנגלית",
volume = "169",
pages = "289--293",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier B.V.",
number = "2",
}