TY - JOUR
T1 - Genetic and functional analysis of genes required for the post-modification of the polyketide antibiotic TA of Myxococcus xanthus
AU - Paitan, Yossi
AU - Orr, Elisha
AU - Ron, Eliora Z.
AU - Rosenberg, Eugene
PY - 1999/11
Y1 - 1999/11
N2 - The antibiotic TA of Myxococcus xanthus is a complex macrocyclic polyketide, produced through successive condensations of acetate by a type I PKS (polyketide synthase) mechanism. The genes encoding TA biosynthesis are clustered on a 36 kb DNA fragment, which has been cloned and analysed. The chemical structure of TA and the mechanism by which it is synthesized indicate the need for several post-modification steps, which are introduced into the carbon chain of the polyketide to form the final bioactive molecule. These include the addition of several carbon atoms originating from acetate carbonyl, three C-methylations, O-methylation and a specific hydroxylation. This paper reports the analysis of five genes which are involved in the postmodification of TA. Their functional analysis, by specific gene disruption, suggests that they may be essential for the production of the active antibiotic. The characteristics and organization of the genes suggest that they may be involved in the addition of the carbon atoms which arise from acetate.
AB - The antibiotic TA of Myxococcus xanthus is a complex macrocyclic polyketide, produced through successive condensations of acetate by a type I PKS (polyketide synthase) mechanism. The genes encoding TA biosynthesis are clustered on a 36 kb DNA fragment, which has been cloned and analysed. The chemical structure of TA and the mechanism by which it is synthesized indicate the need for several post-modification steps, which are introduced into the carbon chain of the polyketide to form the final bioactive molecule. These include the addition of several carbon atoms originating from acetate carbonyl, three C-methylations, O-methylation and a specific hydroxylation. This paper reports the analysis of five genes which are involved in the postmodification of TA. Their functional analysis, by specific gene disruption, suggests that they may be essential for the production of the active antibiotic. The characteristics and organization of the genes suggest that they may be involved in the addition of the carbon atoms which arise from acetate.
KW - Acyl carrier protein
KW - Antibiotic post-modification
KW - Myxococcus xanthus
KW - Polyketide synthase
KW - β-keroacyl ACP synthase III
UR - http://www.scopus.com/inward/record.url?scp=0032716771&partnerID=8YFLogxK
U2 - 10.1099/00221287-145-11-3059
DO - 10.1099/00221287-145-11-3059
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C2 - 10589713
AN - SCOPUS:0032716771
SN - 1350-0872
VL - 145
SP - 3059
EP - 3067
JO - Microbiology
JF - Microbiology
IS - 11
ER -