@inbook{01878264cd174666be94c7cb48f28cb0,
title = "Generation of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated pheS for Counterselection",
abstract = "Gene alteration/deletion by allelic exchange is the preferred strategy for gene manipulation in bacteria. Here we present the fundamentals for an efficient allelic exchange gene deletion method in the bacterial pathogen Listeria monocytogenes. Combining vector generation by Gibson assembly with a counterselection system based on the mutated phenylalanine synthetase (pheS*) makes the generation of gene deletion mutants straightforward and time efficient.",
keywords = "Allelic exchange, Counterselection, Gibson assembly, Listeria monocytogenes, Mutagenesis, pheS",
author = "{Ran Sapir}, Shai and Etai Boichis and Herskovits, {Anat A.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1971-1_1",
language = "אנגלית",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "3--10",
booktitle = "Methods in Molecular Biology",
}