@article{68150ee15dc64b92ab0d04d7b0f726d8,
title = "Generation of antibodies against prion protein in wild-type mice via helix 1 peptide immunization",
abstract = "We present here the development of antibodies against prion protein in BALB/C mice using as antigen human helix 1 of PrP. This sequence is suggested to be involved in protein pathological conformational changes, and is distinguished from that of mice by one amino acid. The immune tolerance to an 'almost-self' epitope and the poor immunogenicity of short peptides was overcome by using Multiple Antigen Peptide displaying eight copies of helix 1. The generated antibodies recognize the whole prion protein with a high binding constant and the established protocol may lead to an active immunization towards therapeutics of prion disease.",
keywords = "Active immunization, Anti-PrP antibodies, Helix 1, Prion protein",
author = "Michal Arbel and Vered Lavie and Beka Solomon",
note = "Funding Information: We thank Dr. A. Taraboulos and the members of his laboratory for providing of N2a-C10 cell line and performing experiments with the whole prion protein. This work was supported by a generous grant from the Israel Center for the Study of Emerging Diseases.",
year = "2003",
month = nov,
doi = "10.1016/j.jneuroim.2003.08.036",
language = "אנגלית",
volume = "144",
pages = "38--45",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier B.V.",
number = "1-2",
}