Gene integration into bovine sperm genome and its expression in transgenic offspring

Mordechai Shemesh; M. Gurevich; E. Harel-Markowitz; L. Benvenisti; L. S. Shore; Y. Stram

doi:10.1002/(sici)1098-2795(200006)56:2+<306::aid-mrd21>3.0.co;2-3

Gene integration into bovine sperm genome and its expression in transgenic offspring

Mordechai Shemesh^*, M. Gurevich, E. Harel-Markowitz, L. Benvenisti, L. S. Shore, Y. Stram

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Transgenic bovine sperm were produced by restriction enzyme mediated insertion (REMI). REMI utilizes lipofection of linearized pEGFP and the corresponding restriction enzyme for integration into the sperm genomic DNA. The transgenic sperm were used in IVF to produce morula expressing GFP. When transgenic sperm were used for Al in two cows, the resultant calves expressed the exogenous DNA in their lymphocytes as determined by (a) PCR and RT-PCR; (b) specific emission of green fluorescence by GFP; and (c) Southern blot analysis. Data demonstrate that REMI is an efficient method for the production of transgenic sperm and corresponding offspring by Al or embryos by IVF. (C) 2000 Wiley-Liss, Inc.

Original language	English
Pages (from-to)	306-308
Number of pages	3
Journal	Molecular Reproduction and Development
Volume	56
Issue number	2 SUPPL.
DOIs	https://doi.org/10.1002/(sici)1098-2795(200006)56:2+<306::aid-mrd21>3.0.co;2-3
State	Published - 2000
Externally published	Yes

Keywords

Cow
GFP
Morula
Transgenic sperm

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10.1002/(sici)1098-2795(200006)56:2+<306::aid-mrd21>3.0.co;2-3

Cite this

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AU - Harel-Markowitz, E.

AU - Benvenisti, L.

AU - Shore, L. S.

AU - Stram, Y.

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Gene integration into bovine sperm genome and its expression in transgenic offspring

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