GC-made protein disorder sheds new light on vertebrate evolution

Arup Panda, Soumita Podder, Sandip Chakraborty, Tapash Chandra Ghosh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


At the emergence of endothermic vertebrates, GC rich regions of the ectothermic ancestral genomes underwent a significant GC increase. Such an increase was previously postulated to increase thermodynamic and structural stability of proteins through selective increase of protein hydrophobicity. Here, we found that, increase in GC content promotes a higher content of disorder promoting amino acid in endothermic vertebrates proteins and that the increase in hydrophobicity is mainly due to a higher content of the small disorder promoting amino acid alanine. In endothermic vertebrates, prevalence of disordered residues was found to promote functional diversity of proteins encoded by GC rich genes. Higher fraction of disordered residues in this group of proteins was also found to minimize their aggregation tendency. Thus, we propose that the GC transition has favored disordered residues to promote functional diversity in GC rich genes, and to protect them against functional loss by protein misfolding.

Original languageEnglish
Pages (from-to)530-537
Number of pages8
Issue number6
StatePublished - 1 Dec 2014
Externally publishedYes


  • GC transition
  • Isochores
  • Protein hydrophobicity
  • Protein intrinsic disorder content
  • Vertebrate evolution


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