GABA controls the level of quorum-sensing signal in Agrobacterium tumefaciens

Romain Chevrot, Ran Rosen, Elise Haudecoeur, Amélie Cirou, Barry J. Shelp, Eliora Ron, Denis Faure

Research output: Contribution to journalArticlepeer-review

Abstract

The concentration of GABA increases rapidly in wounded plant tissues, but the implication of this GABA pulse for plant-bacteria interactions is not known. Here we reveal that GABA stimulated the inactivation of the N-(3-oxooctanoyl)homoserine lactone (OC8-HSL) quorum-sensing signal (or "quormone") by the Agrobacterium lactonase AttM. GABA induced the expression of the attKLM operon, which was correlated to a decrease in OC8-HSL concentration in Agrobacterium tumefaciens cultures. The Agrobacterium GABA transporter Bra was required for this GABA-signaling pathway. Furthermore, transgenic tobacco plants with elevated GABA levels were less sensitive to A. tumefaciens C58 infection than were wild-type plants. These findings indicate that plant GABA may modulate quorum sensing in A. tumefaciens, thereby affecting its virulence on plants. Whereas GABA is an essential cell-to-cell signal in eukaryotes, here we provide evidence of GABA acting as a signal between eukaryotes and pathogenic bacteria. The GABA signal represents a potential target for the development of a strategy to control the virulence of bacterial pathogens.

Original languageEnglish
Pages (from-to)7460-7464
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number19
DOIs
StatePublished - 9 May 2006
Externally publishedYes

Keywords

  • Lactonase
  • Phytopathology
  • Plant signal
  • Quorum quenching

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