TY - JOUR
T1 - G protein-activated K+ channels
T2 - A reporter for rapid activation of G proteins by lysophosphatidic acid in Xenopus oocytes
AU - Van-Ham, Irit Itzhaki
AU - Peleg, Sagit
AU - Dascal, Nathan
AU - Shapira, Hagit
AU - Oron, Yoram
N1 - Funding Information:
This work was supported in part by grants from the Bi-National Science Foundation to Y.O. and N.D., and NIH Grant RO1 GM 58493 to N.D. The work presented in this paper is in partial fulfillment of the requirements for a PhD thesis of I.I.V.-H. at the Sackler Faculty of Medicine, Tel Aviv University.
PY - 2004/4/23
Y1 - 2004/4/23
N2 - Threshold concentrations of lysophosphatidic acid (LPA) or acetylcholine (ACh) induce pertussis toxin (PTX)-sensitive rapid desensitization of responses to LPA in Xenopus oocytes. To demonstrate that threshold [LPA] rapidly activates Gi/o proteins, we used the G protein-activated K+ channel (GIRK) as a reporter. Low [LPA] induced IK+ in <3 s of the agonist addition with little or no activation of chloride current. Depletion of Gαo/Gαo1 each decreased the LPA-induced IK+ by approximately 40-50%, while PTX completely abolished it. This is the first direct evidence showing the activation of GIRK by LPA, and the involvement of G proteins of the Go family in rapid desensitization of LPA responses.
AB - Threshold concentrations of lysophosphatidic acid (LPA) or acetylcholine (ACh) induce pertussis toxin (PTX)-sensitive rapid desensitization of responses to LPA in Xenopus oocytes. To demonstrate that threshold [LPA] rapidly activates Gi/o proteins, we used the G protein-activated K+ channel (GIRK) as a reporter. Low [LPA] induced IK+ in <3 s of the agonist addition with little or no activation of chloride current. Depletion of Gαo/Gαo1 each decreased the LPA-induced IK+ by approximately 40-50%, while PTX completely abolished it. This is the first direct evidence showing the activation of GIRK by LPA, and the involvement of G proteins of the Go family in rapid desensitization of LPA responses.
KW - ASODN, antisense oligodeoxynucleotide
KW - G protein-activated potassium channel
KW - GIRK, G protein-activated K channel
KW - Go/i protein
KW - LPA, lysophosphatidic acid
KW - Lysophosphatidic acid receptor
KW - Rapid desensitization
UR - http://www.scopus.com/inward/record.url?scp=1842828888&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(04)00334-5
DO - 10.1016/S0014-5793(04)00334-5
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AN - SCOPUS:1842828888
SN - 0014-5793
VL - 564
SP - 157
EP - 160
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -