i and Gβγ jointly regulate the conformations of a Gβγ effector, the neuronal G protein-activated K+ channel (GIRK)

Shai Berlin*, Tal Keren-Raifman, Ruth Castel, Moran Rubinstein, Carmen W. Dessauer, Tatiana Ivanina, Nathan Dascal

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Stable complexes among G proteins and effectors are an emerging concept in cell signaling. The prototypical Gβγ effector G protein-activated K+ channel (GIRK; Kir3) physically interacts with Gβγ but also with Gαi/o. Whether and how Gαi/o subunits regulate GIRK in vivo is unclear. We studied triple interactions among GIRK subunits 1 and 2, Gαi3 and Gβγ. We used in vitro protein interaction assays and in vivo intramolecular Förster resonance energy transfer (i-FRET) between fluorophores attached to N and C termini of either GIRK1 or GIRK2 subunit. We demonstrate, for the first time, that Gβγ and Gαi3 distinctly and interdependently alter the conformational states of the heterotetrameric GIRK1/2 channel. Biochemical experiments show that Gβγ greatly enhances the binding of GIRK1 subunit to Gαi3GDP and, unexpectedly, to Gαi3GTP. i-FRET showed that both Gαi3 and Gβγ induced distinct conformational changes in GIRK1 and GIRK2. Moreover, GIRK1 and GIRK2 subunits assumed unique, distinct conformations when coexpressed with a "constitutively active" Gαi3 mutant andGβγ together. These conformations differ from those assumed by GIRK1 or GIRK2 after separate coexpression of either Gαi3 or Gβγ. Both biochemical and i-FRET data suggest that GIRK acts as the nucleator of the GIRK-Gα-Gβγ signaling complex and mediates allosteric interactions between GαiGTP and Gβγ. Our findings imply that Gαi/o and the Gαiβγ heterotrimer can regulate a Gβγ effector both before and after activation by neurotransmitters.

Original languageEnglish
Pages (from-to)6179-6185
Number of pages7
JournalJournal of Biological Chemistry
Issue number9
StatePublished - 26 Feb 2010


FundersFunder number
National Institute of General Medical SciencesR01GM068493


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