11 and Gαq guaninine nucleotide regulatory proteins differentially modulate the response to thyrotropin-releasing hormone in Xenopus oocytes

Dafna Lipinsky, Marvin C. Gershengorn, Yoram Oron

Research output: Contribution to journalArticlepeer-review

Abstract

Xenopus oocytes that express mouse thyrotropin-releasing hormone receptors (TRH-Rs) after injection if RNA transcribed from TRH-R cDNA respond to THR by a depolarizing current. This response is transducer by activation of phosphoinositide-specific phospholipase C and utilizes an as yet unidentified endogenous guanine nucleotide-binding regulatory (G) protein(s). The α subunit of G11 and Gq have recently been shown to couple receptors to activation of phospholipase C. To determine whether there are functional differences between these proteins, we have co-expressed the TRH-R with either α11 or αq, α11 potentiated the response to TRH (by 61±16%), while αq inhibited the response (by 37±9%). The changes in amplitudes were accompanied by inverse changes in response latencies. These data show that α11 and αq differentially modulate signal transduction in Xenopus oocytes.

Original languageEnglish
Pages (from-to)237-240
Number of pages4
JournalFEBS Letters
Volume307
Issue number2
DOIs
StatePublished - 28 Jul 1992

Keywords

  • G-protein
  • TRH receptor
  • Xenopus oocyte

Fingerprint

Dive into the research topics of 'Gα11 and Gαq guaninine nucleotide regulatory proteins differentially modulate the response to thyrotropin-releasing hormone in Xenopus oocytes'. Together they form a unique fingerprint.

Cite this