11 and Gαq guaninine nucleotide regulatory proteins differentially modulate the response to thyrotropin-releasing hormone in Xenopus oocytes

Dafna Lipinsky, Marvin C. Gershengorn, Yoram Oron*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Xenopus oocytes that express mouse thyrotropin-releasing hormone receptors (TRH-Rs) after injection if RNA transcribed from TRH-R cDNA respond to THR by a depolarizing current. This response is transducer by activation of phosphoinositide-specific phospholipase C and utilizes an as yet unidentified endogenous guanine nucleotide-binding regulatory (G) protein(s). The α subunit of G11 and Gq have recently been shown to couple receptors to activation of phospholipase C. To determine whether there are functional differences between these proteins, we have co-expressed the TRH-R with either α11 or αq, α11 potentiated the response to TRH (by 61±16%), while αq inhibited the response (by 37±9%). The changes in amplitudes were accompanied by inverse changes in response latencies. These data show that α11 and αq differentially modulate signal transduction in Xenopus oocytes.

Original languageEnglish
Pages (from-to)237-240
Number of pages4
JournalFEBS Letters
Volume307
Issue number2
DOIs
StatePublished - 28 Jul 1992

Funding

FundersFunder number
US-Israel Binational Science Foundation

    Keywords

    • G-protein
    • TRH receptor
    • Xenopus oocyte

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