TY - JOUR
T1 - Further enhancement of baculovirus insecticidal efficacy with scorpion toxins that interact cooperatively
AU - Regev, Avital
AU - Rivkin, Hadassah
AU - Inceoglu, Bora
AU - Gershburg, Eduard
AU - Hammock, Bruce D.
AU - Gurevitz, Michael
AU - Chejanovsky, Nor
N1 - Funding Information:
This work was supported by grants IS-2530-95C (N.C., BDH) and IS-2901-97C (M.G.) from BARD, The United States-Israel Binational Agricultural Research and Development, from the Dutch–Israeli Agricultural Research Program (DIARP 97/29, N.C., M.G.), and from The Israeli Academy of Science and Humanities (466/97, M.G.). We thank Dr. D. Nestel from the Volcani Center, Israel, for his kind help in statistical analysis of the data. Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel No. 519-02.
PY - 2003/2/27
Y1 - 2003/2/27
N2 - We have studied whether the cooperative insecticidal effect of certain scorpion toxin pairs, namely either a combination of excitatory and depressant, or alpha and depressant scorpion toxins, would improve the efficacy of Autographa californica nucleopolyhedrovirus (AcMNPV) over a virus expressing only a single toxin, towards Heliothis virescens, Helicoverpa armigera, and Spodoptera littoralis larvae. The best result was achieved by combined expression of the excitatory toxin, LqhIT1, and the depressant toxin, LqhIT2, that provided an ET50 value of 46.9 h on H. virescens neonates, an improvement of 40% over the efficacy of wild-type AcMNPV, and of 18% and 22% over baculoviruses that express each of the toxins independently. These results demonstrate that significant improvement in efficacy of recombinant baculoviruses is obtainable with toxins that exhibit a cooperative effect, and may contribute to employ baculoviruses to replace hazardous chemicals in insect control.
AB - We have studied whether the cooperative insecticidal effect of certain scorpion toxin pairs, namely either a combination of excitatory and depressant, or alpha and depressant scorpion toxins, would improve the efficacy of Autographa californica nucleopolyhedrovirus (AcMNPV) over a virus expressing only a single toxin, towards Heliothis virescens, Helicoverpa armigera, and Spodoptera littoralis larvae. The best result was achieved by combined expression of the excitatory toxin, LqhIT1, and the depressant toxin, LqhIT2, that provided an ET50 value of 46.9 h on H. virescens neonates, an improvement of 40% over the efficacy of wild-type AcMNPV, and of 18% and 22% over baculoviruses that express each of the toxins independently. These results demonstrate that significant improvement in efficacy of recombinant baculoviruses is obtainable with toxins that exhibit a cooperative effect, and may contribute to employ baculoviruses to replace hazardous chemicals in insect control.
KW - Cooperative anti-insect scorpion toxin
KW - Helicoverpa armigera
KW - Heliothis virescens
KW - Recombinant baculovirus
KW - Spodoptera littoralis
UR - http://www.scopus.com/inward/record.url?scp=0037468405&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(03)00104-2
DO - 10.1016/S0014-5793(03)00104-2
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AN - SCOPUS:0037468405
SN - 0014-5793
VL - 537
SP - 106
EP - 110
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -