@article{9c9d2d8b8626497dab51564515edd4ee,
title = "Functional study of elafin cleaved by Pseudomonas aeruginosa metalloproteinases",
abstract = "Elafin is a 6-kDa innate immune protein present at several epithelial surfaces including the pulmonary epithelium. It is a canonical protease inhibitor of two neutrophil serine proteases wneutrophil elastase (NE) and proteinase 3x with the capacity to covalently bind extracellular matrix proteins by transglutamination. In addition to these properties, elafin also possesses antimicrobial and immunomodulatory activities. The aim of the present study was to investigate the effect of Pseudomonas aeruginosa proteases on elafin function. We found that P. aeruginosa PAO1-conditioned medium and two purified Pseudomonas metalloproteases, pseudolysin (elastase) and aeruginolysin (alkaline protease), are able to cleave recombinant elafin. Pseudolysin was shown to inactivate the anti-NE activity of elafin by cleaving its protease-binding loop. Interestingly, antibacterial properties of elafin against PAO1 were found to be unaffected after pseudolysin treatment. In contrast to pseudolysin, aeruginolysin failed to inactivate the inhibitory properties of elafin against NE. Aeruginolysin cleaves elafin at the amino-terminal Lys6- Gly7 peptide bond, resulting in a decreased ability to covalently bind purified fibronectin following transglutaminase activity. In conclusion, this study provides evidence that elafin is susceptible to proteolytic cleavage at alternative sites by P. aeruginosa metalloproteinases, which can affect different biological functions of elafin.",
keywords = "Antimicrobials, Antiproteases, Cleavage, Microbial, Proteases, Transglutaminase",
author = "Nicolas Guyot and Gudmundur Bergsson and Butler, {Marcus W.} and Greene, {Catherine M.} and Sin{\'e}ad Weldon and Efrat Kessler and Levine, {Rodney L.} and O'Neill, {Shane J.} and Taggart, {Clifford C.} and McElvaney, {Noel G.}",
note = "Funding Information: This work was supported by the Health Research Board, Alpha One Foundation, Programme for Research in Third levels Institutes administered by Higher Education Authority, Science Foundation Ireland, Cystic Fibrosis Hope Source, Cystic Fibrosis Research Trust, Cystic Fibrosis Association of Ireland (awarded to C.T.) and the Royal College of Surgeons in Ireland.",
year = "2010",
month = jun,
doi = "10.1515/BC.2010.066",
language = "אנגלית",
volume = "391",
pages = "705--716",
journal = "Biological Chemistry",
issn = "1431-6730",
publisher = "Walter de Gruyter GmbH",
number = "6",
}