Functional expression of the Candida albicans β-tubulin gene in Saccharomyces cerevisiae

Herbert A. Smith; John W. Gorman; Yigal Koltin; Jessica A. Gorman

doi:10.1016/0378-1119(90)90446-X

Functional expression of the Candida albicans β-tubulin gene in Saccharomyces cerevisiae

Herbert A. Smith, John W. Gorman, Yigal Koltin, Jessica A. Gorman^*

^*Corresponding author for this work

Biotechnology

GlaxoSmithKline

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11 Scopus citations

Abstract

Expression of the β-tubulin-encoding gene (TUB2) of Candida albicans has been examined in Saccharomyces cerevisiae. Overexpression of the TUB2 gene of C. albicans, as well as that of S. cerevisiae, was found to be lethal. Chromosomal integration of the C. albicans TUB2 gene into a strain in which the native TUB2 gene had been deleted led to functional complementation. The results demonstrate that correct splicing of the two introns present in the C. albicans TUB2 gene occurs in the heterologous host strain containing this gene. Such strains are supersensitive to the tubulin-binding agent benomyl, indicating that the natural resistance of C. albicans to benomyl is not related to the structure of its β-tubulin.

Original language	English
Pages (from-to)	115-123
Number of pages	9
Journal	Gene
Volume	90
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(90)90446-X
State	Published - 31 May 1990

Keywords

RNA splicing
Recombinant DNA
benomyl
complementation
intron
microtubules

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10.1016/0378-1119(90)90446-X

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title = "Functional expression of the Candida albicans β-tubulin gene in Saccharomyces cerevisiae",

abstract = "Expression of the β-tubulin-encoding gene (TUB2) of Candida albicans has been examined in Saccharomyces cerevisiae. Overexpression of the TUB2 gene of C. albicans, as well as that of S. cerevisiae, was found to be lethal. Chromosomal integration of the C. albicans TUB2 gene into a strain in which the native TUB2 gene had been deleted led to functional complementation. The results demonstrate that correct splicing of the two introns present in the C. albicans TUB2 gene occurs in the heterologous host strain containing this gene. Such strains are supersensitive to the tubulin-binding agent benomyl, indicating that the natural resistance of C. albicans to benomyl is not related to the structure of its β-tubulin.",

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AU - Smith, Herbert A.

AU - Gorman, John W.

AU - Koltin, Yigal

AU - Gorman, Jessica A.

PY - 1990/5/31

Y1 - 1990/5/31

N2 - Expression of the β-tubulin-encoding gene (TUB2) of Candida albicans has been examined in Saccharomyces cerevisiae. Overexpression of the TUB2 gene of C. albicans, as well as that of S. cerevisiae, was found to be lethal. Chromosomal integration of the C. albicans TUB2 gene into a strain in which the native TUB2 gene had been deleted led to functional complementation. The results demonstrate that correct splicing of the two introns present in the C. albicans TUB2 gene occurs in the heterologous host strain containing this gene. Such strains are supersensitive to the tubulin-binding agent benomyl, indicating that the natural resistance of C. albicans to benomyl is not related to the structure of its β-tubulin.

AB - Expression of the β-tubulin-encoding gene (TUB2) of Candida albicans has been examined in Saccharomyces cerevisiae. Overexpression of the TUB2 gene of C. albicans, as well as that of S. cerevisiae, was found to be lethal. Chromosomal integration of the C. albicans TUB2 gene into a strain in which the native TUB2 gene had been deleted led to functional complementation. The results demonstrate that correct splicing of the two introns present in the C. albicans TUB2 gene occurs in the heterologous host strain containing this gene. Such strains are supersensitive to the tubulin-binding agent benomyl, indicating that the natural resistance of C. albicans to benomyl is not related to the structure of its β-tubulin.

KW - RNA splicing

KW - Recombinant DNA

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KW - complementation

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Functional expression of the Candida albicans β-tubulin gene in Saccharomyces cerevisiae

Abstract

Keywords

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