Functional expression of the Candida albicans β-tubulin gene in Saccharomyces cerevisiae

Herbert A. Smith, John W. Gorman, Yigal Koltin, Jessica A. Gorman

Research output: Contribution to journalArticlepeer-review

Abstract

Expression of the β-tubulin-encoding gene (TUB2) of Candida albicans has been examined in Saccharomyces cerevisiae. Overexpression of the TUB2 gene of C. albicans, as well as that of S. cerevisiae, was found to be lethal. Chromosomal integration of the C. albicans TUB2 gene into a strain in which the native TUB2 gene had been deleted led to functional complementation. The results demonstrate that correct splicing of the two introns present in the C. albicans TUB2 gene occurs in the heterologous host strain containing this gene. Such strains are supersensitive to the tubulin-binding agent benomyl, indicating that the natural resistance of C. albicans to benomyl is not related to the structure of its β-tubulin.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalGene
Volume90
Issue number1
DOIs
StatePublished - 31 May 1990

Keywords

  • RNA splicing
  • Recombinant DNA
  • benomyl
  • complementation
  • intron
  • microtubules

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