@article{5b8a2c7a2c70458aa563c0fdb6def2d8,
title = "Functional expression of an alpha anti-insect scorpion neurotoxin in insect cells and lepidopterous larvae",
abstract = "The Leiurus quinquestriatus hebraeus alpha anti-insect toxin (LqhαIT) cDNA was engineered into the Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. Insect cells infected with the recombinant virus secreted a functional LqhαIT polypeptide. Spodoptera littoralis and Heliothis armigera larvae injected with recombinant budded virus, showed typical intoxication symptoms. This recombinant virus showed enhanced insecticidal potency against H. armigera larvae compared with wild type AcNPV. The present expression system will facilitate: (1) the future elucidation of structural elements involved in its prominent anti-insect toxicity; and (2) the future design of genetically modified alpha toxins with improved anti-insect selectivity.",
keywords = "Baculovirus, Insect cells, Lepidopterous larvae, LqhαIT (Leiurus quinquestriatus hebraeus) anti-insect toxin",
author = "Nor Chejanovsky and Noam Zilberberg and Hadasah Rivkin and Eliahu Zlotkin and Michael Gurevitz",
note = "Funding Information: Acknowledgements. This work was supported by grants IS-2139-92 (N.C.) and IS-1982 91 (M.G), from BARD, the United States-Israel Binational Agricultural Research & Development Fund and No. 93-00924 from US-Israel Binational science Foundation (BSF) (E.Z.).",
year = "1995",
month = dec,
day = "4",
doi = "10.1016/0014-5793(95)01263-4",
language = "אנגלית",
volume = "376",
pages = "181--184",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "3",
}