TY - JOUR
T1 - Functional conservation of Dhh1p, a cytoplasmic DExD/H-box protein present in large complexes
AU - Tseng-Rogenski, Stephanie S.I.
AU - Chong, Jean Leon
AU - Thomas, Christopher B.
AU - Enomoto, Shinichiro
AU - Berman, Judith
AU - Chang, Tien Hsien
N1 - Funding Information:
We thank J. Sommerville for the Xp54 cDNA clone; R.-Y. Chuang and P. Herman for sage advice; L. Tung and R. Hage for commenting on the manuscript. C.B.T. was supported by a NSF-REU grant to the Department of Molecular Genetics, The Ohio State University. T.-H.C. has been supported by NIH (GM48752) and NSF (MCB-9982726). J.B. has been supported by NIH (GM38626).
PY - 2003/9/1
Y1 - 2003/9/1
N2 - The DHH1 gene in the yeast Saccharomyces cerevisiae encodes a putative RNA helicase of remarkable sequence similarity to several other DExD/H-box proteins, including Xp54 in Xenopus laevis and Ste13p in Schizosaccharomyces pombe. We show here that over-expression of Xp54, an integral component of the stored messenger ribonucleoprotein (mRNP) particles, can rescue the loss of Dhh1p in yeast. Localization and sedimentation studies showed that Dhh1p exists predominantly in the cytoplasm and is present in large complexes whose sizes appear to vary according to the growth stage of the cell culture. In addition, deletion of dhh1, when placed in conjunction with the mutant dbP5 and ded1 alleles, resulted in a synergistically lethal effect, suggesting that Dhh1p may have a role in mRNA export and translation. Finally, similar to Ste13p, Dhh1p is required for sporulation in the budding yeast. Taken together, our data provide evidence that the functions of Dhh1p are conserved through evolution.
AB - The DHH1 gene in the yeast Saccharomyces cerevisiae encodes a putative RNA helicase of remarkable sequence similarity to several other DExD/H-box proteins, including Xp54 in Xenopus laevis and Ste13p in Schizosaccharomyces pombe. We show here that over-expression of Xp54, an integral component of the stored messenger ribonucleoprotein (mRNP) particles, can rescue the loss of Dhh1p in yeast. Localization and sedimentation studies showed that Dhh1p exists predominantly in the cytoplasm and is present in large complexes whose sizes appear to vary according to the growth stage of the cell culture. In addition, deletion of dhh1, when placed in conjunction with the mutant dbP5 and ded1 alleles, resulted in a synergistically lethal effect, suggesting that Dhh1p may have a role in mRNA export and translation. Finally, similar to Ste13p, Dhh1p is required for sporulation in the budding yeast. Taken together, our data provide evidence that the functions of Dhh1p are conserved through evolution.
UR - http://www.scopus.com/inward/record.url?scp=0344875482&partnerID=8YFLogxK
U2 - 10.1093/nar/gkg712
DO - 10.1093/nar/gkg712
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0344875482
VL - 31
SP - 4995
EP - 5002
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 17
ER -