Functional characterization of mongoose nicotinic acetylcholine receptor α-subunit: Resistance to α-bungarotoxin and high sensitivity to acetylcholine

Orna Asher, Monica Lupu-Meiri, Bo S. Jensen, Tamar Paperna, Sara Fuchs, Yoram Oron

Research output: Contribution to journalArticlepeer-review

Abstract

The mongoose is resistant to snake neurotoxins. The mongoose muscle nicotine acetylcholine receptor (AChR) α-subunit contains a number of mutations in the ligand-binding domain and exhibits poor binding of α-bungarotoxin (α-BTX). We characterized the functional properties of a hybrid (α-mongoose/βγδ-rat) AChR. Hybrid AChRs, expressed in Xenopus oocytes, respond to acetylcholine with depolarizing current, the mean maximal amplitude of which was greater than that mediated by the rat AChR. The IC50 of α-BTX to the hybrid AChR was 200-fold greater than that of the rat, suggesting much lower affinity for the toxin. Hybrid AChRs exhibited an apparent higher rate of desensitization and higher affinity for ACh (EC50 1.3 vs. 23.3 μM for the rat AChR). Hence, changes in the ligand-binding domain of AChR not only affect the binding properties of the receptor, but also result in marked changes in the characteristics of the current.

Original languageEnglish
Pages (from-to)411-414
Number of pages4
JournalFEBS Letters
Volume431
Issue number3
DOIs
StatePublished - 24 Jul 1998

Keywords

  • Acetylcholine
  • Acetylcholine receptor
  • Mongoose
  • Xenopus oocyte
  • α-Bungarotoxin

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