Fructose and myo-inositol paper chromatography in seminal and prostatic fluid samples. A critical evaluation

Y. Soffer, D. Pace, E. Caspi, L. M. Lewin

Research output: Contribution to journalArticlepeer-review


Fructose and myo-inositol paper chromatography (FMPC) has been performed in more than 400 seminal and 80 prostatic fluid samples, as a screening method for male accessory gland dysfunction. Fructose was also estimated by the resorcinol colorimetric method in 103 cases. In 256 cases seminal cations (Zn++, Mg++ and/or Ca++) were estimated by atomic absorption spectrophotometry. In 95 cases seminal acid phosphatase was estimated. Fructose chromatographic evaluation was compared to the resorcinol quantitative estimation and myo-inositol to cations and acid phosphatase estimations. FMPC agreed with colorimetric fructose determination in 93% of the samples tested and appears to be a useful and reliable screening method for seminal vesicle function in semen. Low myo-inositol concentration in prostatic fluid correlates well with low Zn++ concentration. FMPC in prostatic fluid is a useful means for screening patients for prostatic function although it is somewhat less sensitive than measurement of seminal Zn++. Comparison of seminal prostatic markers shows that Zn++ concentrations are better indicators of prostatic dysfunction than is myo inositol in seminal fluid. Acid phosphatase determinations were less reliable indicators than were cation determinations or myo inositol chromatography. FMPC, which requires minimal equipment and aliquots of no more than 5 μl of seminal or prostatic fluid, detects accessory gland dysfunction satisfactorily and is useful for andrological evaluation.

Original languageEnglish
Pages (from-to)271-284
Number of pages14
Issue number3
StatePublished - 1979


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