Fringe mode transmittance laser Doppler microscope anemometer: its adaptation for measurement in the microcirculation

S. Einav*, H. J. Berman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Blood flow analysis in the microcirculation requires accurate measurement of velocity, volume flow and shear-rate versus shear-stress relationships. The resolution of most anemometers is too limited to obtain useful measurements, especially near the blood vessel wall and at branches and bifurcations. To make such measurements possible with a noninvasive, high resolution, accurate technique, we have developed a fringe mode, transmittance laser Doppler microscope anemometer (LDMA). This system has an intrinsically high spatial resolution (10 × 12 μm), and does not require a high concentration (106/cm3) of scatterers or red blood cells (RBC) as in our application. Preliminary measurements of water flow in a rectangular channel were conducted to ascertain the reliability and accuracy of velocity measurements using the LDMA. Velocity profiles were then measured by the LDMA system in arterioles 38-135 μm in diameter, in the transparent, everted cheek pouch of the anaesthelized hamster. The extremely high resolution of the optical system, and the ultra-fine traversing mechanism of the microscope slage, made velocity readings larger than 0.02 mm/s with accuracy and reproducibility better than 1%, possible near the wall to within 7-10 μm.

Original languageEnglish
Pages (from-to)393-399
Number of pages7
JournalJournal of Biomedical Engineering
Volume10
Issue number5
DOIs
StatePublished - Oct 1988

Keywords

  • Circulatory system
  • blood flow
  • laser Doppler anemometry

Fingerprint

Dive into the research topics of 'Fringe mode transmittance laser Doppler microscope anemometer: its adaptation for measurement in the microcirculation'. Together they form a unique fingerprint.

Cite this