This study evaluated the impact of sperm cryopreservation on sperm quality. The HZA was used to test the binding capacity of fresh as opposed to frozen-thawed sperm from 12 donors. Fresh and frozen-thawed sperm motility was 47% ± 1.5% and 24% ± 3.8% (mean ± SE), respectively. However, the number of sperm cells attached to the hemizonae was 75 ± 12.0 and 74 ± 11.9, respectively. We conclude that cryopreservation results in a reduced number of motile sperm cells but does not adversely affect the ability of rescued sperm cells to bind to the ZP. The study also supports the use of frozen-thawed rather than fresh donor sperm for control in the HZA procedure.
- hemizona assay
- zona pellucida