@article{b7276c77314c42ce8a85b3bc570035fa,
title = "Fractionation of mast cell components for studies of ligand-receptor binding at the plasma membrane",
abstract = "This communication describes a simple procedure for fractionating mast cells producing plasma membranes and intact granules. Mast cells were purified over a bovine serum albumin density gradient and disrupted under conditions in which no histamine was released. Iodinated immunoglobulin E (IgE) bound to the cells served as a marker for the plasma membrane fraction. Employing a discontinuous sucrose gradient the plasma membrane and granule fractions were separated. The specific activity of the IgE binding to the isolated plasma membrane fractions was 10-fold higher compared with that of the IgE binding to intact cells.",
keywords = "IgE-receptor, fractionation, granules, mast cells, plasma membranes",
author = "R. Sagi-Eisenberg and Foreman, {J. C.}",
note = "Funding Information: R. S.-E. was in receipt of a Travelling Fellowship on the Israel Academy of Sciences Royal Society programme. A grant from the Wellcome Trust supported part of this study. We should like to thank Dr. Jocelyn M. Baldwin for her advice and help during this study.",
year = "1984",
doi = "10.1016/0165-2478(84)90103-2",
language = "אנגלית",
volume = "8",
pages = "43--47",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier B.V.",
number = "1",
}