Fractionation of mast cell components for studies of ligand-receptor binding at the plasma membrane

R. Sagi-Eisenberg*, J. C. Foreman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

This communication describes a simple procedure for fractionating mast cells producing plasma membranes and intact granules. Mast cells were purified over a bovine serum albumin density gradient and disrupted under conditions in which no histamine was released. Iodinated immunoglobulin E (IgE) bound to the cells served as a marker for the plasma membrane fraction. Employing a discontinuous sucrose gradient the plasma membrane and granule fractions were separated. The specific activity of the IgE binding to the isolated plasma membrane fractions was 10-fold higher compared with that of the IgE binding to intact cells.

Original languageEnglish
Pages (from-to)43-47
Number of pages5
JournalImmunology Letters
Volume8
Issue number1
DOIs
StatePublished - 1984
Externally publishedYes

Funding

FundersFunder number
Wellcome Trust

    Keywords

    • IgE-receptor
    • fractionation
    • granules
    • mast cells
    • plasma membranes

    Fingerprint

    Dive into the research topics of 'Fractionation of mast cell components for studies of ligand-receptor binding at the plasma membrane'. Together they form a unique fingerprint.

    Cite this