Fourier transform infrared spectra of aqueous protein mixtures using a novel attenuated total internal reflectance cell with infrared fibers

Research output: Contribution to journalArticlepeer-review

Abstract

A specially designed cell containing a silver halide (AgBr:AgCl) infrared fiber allows convenient and reproducible loading of viscous protein solutions and suspensions. Attenuated total internal reflectance measurements using an FTIR spectrometer were made for bovine serum albumin-water paste. Dynamic changes in the protein films are readily followed, a technique which should be generally useful. A band assigned to a secondary structural feature, the α-helix, is similar in intensity to that reported (T.M.Fong, M.G.McNamee, submitted for publication).

Original languageEnglish
Pages (from-to)1059-1063
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume142
Issue number3
DOIs
StatePublished - 13 Feb 1987

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