TY - JOUR
T1 - Fold-Sensitive Visible Fluorescence in β-Sheet Peptide Structures
AU - Apter, Boris
AU - Lapshina, Nadezda
AU - Lapsker, Igor
AU - Handelman, Amir
AU - Accardo, Antonella
AU - Diaferia, Carlo
AU - Morelli, Giancarlo
AU - Rosenman, Gil
N1 - Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/12/3
Y1 - 2021/12/3
N2 - Fluorescence (FL) is a basic optical phenomenon that is widely used in molecular spectroscopy, high-resolution microscopy, and bioimaging. It has exciting and emission spectra defined by an electronic structure of particular fluorochrome molecules. In this work, another type of FL is investigated. This FL phenomenon is observed both in biological amyloid and bioinspired amyloidogenic assemblies, folded into specific biomolecular β-sheet secondary structure. A fine mechanism of this fold-sensitive FL effect, arising at the earliest stages of seeding and nucleation of β-sheet nanofibers, and its growth under thermally activated conformational refolding of helical to β-sheet state in bioinspired ultrashort peptide structures are studied. This FL effect demonstrates different properties compared to the classical FL from non-folded biomolecules or molecular dyes. Its excitation and emission spectra are similar in any β-sheet peptide/protein assemblies irrespective of their biochemical composition, primary structure, and origin. It is shown that this fold-sensitive FL effect exhibits a wideband spectrum, covering the entire visible region (400–650 nm), high quantum yield, and tunable FL wavelength. This research paves the way for advanced FL optical nanomaterials, for new methods of bioimaging and diagnostics as well as for the development of biocompatible nanoscale FL sources.
AB - Fluorescence (FL) is a basic optical phenomenon that is widely used in molecular spectroscopy, high-resolution microscopy, and bioimaging. It has exciting and emission spectra defined by an electronic structure of particular fluorochrome molecules. In this work, another type of FL is investigated. This FL phenomenon is observed both in biological amyloid and bioinspired amyloidogenic assemblies, folded into specific biomolecular β-sheet secondary structure. A fine mechanism of this fold-sensitive FL effect, arising at the earliest stages of seeding and nucleation of β-sheet nanofibers, and its growth under thermally activated conformational refolding of helical to β-sheet state in bioinspired ultrashort peptide structures are studied. This FL effect demonstrates different properties compared to the classical FL from non-folded biomolecules or molecular dyes. Its excitation and emission spectra are similar in any β-sheet peptide/protein assemblies irrespective of their biochemical composition, primary structure, and origin. It is shown that this fold-sensitive FL effect exhibits a wideband spectrum, covering the entire visible region (400–650 nm), high quantum yield, and tunable FL wavelength. This research paves the way for advanced FL optical nanomaterials, for new methods of bioimaging and diagnostics as well as for the development of biocompatible nanoscale FL sources.
KW - bioinspired fluorescent nanomaterials
KW - fold-sensitive fluorescence
KW - functional fluorescence applications
KW - helical conformational state, thermally activated refolding
KW - β-sheet conformational state, β-sheet fibers
UR - http://www.scopus.com/inward/record.url?scp=85102631711&partnerID=8YFLogxK
U2 - 10.1002/adom.202002247
DO - 10.1002/adom.202002247
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AN - SCOPUS:85102631711
SN - 2195-1071
VL - 9
JO - Advanced Optical Materials
JF - Advanced Optical Materials
IS - 23
M1 - 2002247
ER -