Fluorescent in situ hybridization for the detection of t(8:14) in Burkitt's lymphoma

M. Lishner; G. Kenet; A. Lalkin; S. Yarkoni; H. Ben-Bassat; M. Fejgin; G. Rechavi; A. Amiel

doi:10.1159/000204455

Fluorescent in situ hybridization for the detection of t(8:14) in Burkitt's lymphoma

M. Lishner^*, G. Kenet, A. Lalkin, S. Yarkoni, H. Ben-Bassat, M. Fejgin, G. Rechavi, A. Amiel

^*Corresponding author for this work

Hematology

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Abstract

Burkitt’s lymphoma cells exhibit the reciprocal balanced chromosomal trans-location t(8:14) in 75% of patients. Cytogenetic analysis is time-consuming, re-quires in vitro culture with mitogens and enables to analyse a relatively small number of cells. We evaluated the role of multicolor fluorescent in situ hybridization (FISH) in the rapid detection of t(8:14) in Burkitt’s lymphoma cells. FISH detected the JH/myc translocation in 100% of the cells of five cell lines carrying the classical t(8:14) and in fresh cells obtained from a newly diagnosed Burkitt’s lymphoma patient. In contrast, the translocation was not detected in the Bajb cell line that does not carry t(8:14). We conclude that FISH is a rapid and reliable diagnostic tool for the detection of the J_H/myc translocation in Burkitt’s lymphoma patients.

Original language	English
Pages (from-to)	186-189
Number of pages	4
Journal	Acta Haematologica
Volume	90
Issue number	4
DOIs	https://doi.org/10.1159/000204455
State	Published - 1993

Keywords

Burkitt’s lymphoma
Fluorescent in situ hybridization t(8:14)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1159/000204455

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title = "Fluorescent in situ hybridization for the detection of t(8:14) in Burkitt's lymphoma",

abstract = "Burkitt{\textquoteright}s lymphoma cells exhibit the reciprocal balanced chromosomal trans-location t(8:14) in 75% of patients. Cytogenetic analysis is time-consuming, re-quires in vitro culture with mitogens and enables to analyse a relatively small number of cells. We evaluated the role of multicolor fluorescent in situ hybridization (FISH) in the rapid detection of t(8:14) in Burkitt{\textquoteright}s lymphoma cells. FISH detected the JH/myc translocation in 100% of the cells of five cell lines carrying the classical t(8:14) and in fresh cells obtained from a newly diagnosed Burkitt{\textquoteright}s lymphoma patient. In contrast, the translocation was not detected in the Bajb cell line that does not carry t(8:14). We conclude that FISH is a rapid and reliable diagnostic tool for the detection of the JH/myc translocation in Burkitt{\textquoteright}s lymphoma patients.",

keywords = "Burkitt{\textquoteright}s lymphoma, Fluorescent in situ hybridization t(8:14)",

author = "M. Lishner and G. Kenet and A. Lalkin and S. Yarkoni and H. Ben-Bassat and M. Fejgin and G. Rechavi and A. Amiel",

year = "1993",

doi = "10.1159/000204455",

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AU - Lishner, M.

AU - Kenet, G.

AU - Lalkin, A.

AU - Yarkoni, S.

AU - Ben-Bassat, H.

AU - Fejgin, M.

AU - Rechavi, G.

AU - Amiel, A.

PY - 1993

Y1 - 1993

N2 - Burkitt’s lymphoma cells exhibit the reciprocal balanced chromosomal trans-location t(8:14) in 75% of patients. Cytogenetic analysis is time-consuming, re-quires in vitro culture with mitogens and enables to analyse a relatively small number of cells. We evaluated the role of multicolor fluorescent in situ hybridization (FISH) in the rapid detection of t(8:14) in Burkitt’s lymphoma cells. FISH detected the JH/myc translocation in 100% of the cells of five cell lines carrying the classical t(8:14) and in fresh cells obtained from a newly diagnosed Burkitt’s lymphoma patient. In contrast, the translocation was not detected in the Bajb cell line that does not carry t(8:14). We conclude that FISH is a rapid and reliable diagnostic tool for the detection of the JH/myc translocation in Burkitt’s lymphoma patients.

AB - Burkitt’s lymphoma cells exhibit the reciprocal balanced chromosomal trans-location t(8:14) in 75% of patients. Cytogenetic analysis is time-consuming, re-quires in vitro culture with mitogens and enables to analyse a relatively small number of cells. We evaluated the role of multicolor fluorescent in situ hybridization (FISH) in the rapid detection of t(8:14) in Burkitt’s lymphoma cells. FISH detected the JH/myc translocation in 100% of the cells of five cell lines carrying the classical t(8:14) and in fresh cells obtained from a newly diagnosed Burkitt’s lymphoma patient. In contrast, the translocation was not detected in the Bajb cell line that does not carry t(8:14). We conclude that FISH is a rapid and reliable diagnostic tool for the detection of the JH/myc translocation in Burkitt’s lymphoma patients.

KW - Burkitt’s lymphoma

KW - Fluorescent in situ hybridization t(8:14)

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Fluorescent in situ hybridization for the detection of t(8:14) in Burkitt's lymphoma

Abstract

Keywords

UN SDGs

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