Fluorescent in situ hybridization for the detection of t(8:14) in Burkitt's lymphoma

M. Lishner, G. Kenet, A. Lalkin, S. Yarkoni, H. Ben-Bassat, M. Fejgin, G. Rechavi, A. Amiel

Research output: Contribution to journalArticlepeer-review

Abstract

Burkitt’s lymphoma cells exhibit the reciprocal balanced chromosomal trans-location t(8:14) in 75% of patients. Cytogenetic analysis is time-consuming, re-quires in vitro culture with mitogens and enables to analyse a relatively small number of cells. We evaluated the role of multicolor fluorescent in situ hybridization (FISH) in the rapid detection of t(8:14) in Burkitt’s lymphoma cells. FISH detected the JH/myc translocation in 100% of the cells of five cell lines carrying the classical t(8:14) and in fresh cells obtained from a newly diagnosed Burkitt’s lymphoma patient. In contrast, the translocation was not detected in the Bajb cell line that does not carry t(8:14). We conclude that FISH is a rapid and reliable diagnostic tool for the detection of the JH/myc translocation in Burkitt’s lymphoma patients.

Original languageEnglish
Pages (from-to)186-189
Number of pages4
JournalActa Haematologica
Volume90
Issue number4
DOIs
StatePublished - 1993

Keywords

  • Burkitt’s lymphoma
  • Fluorescent in situ hybridization t(8:14)

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