TY - JOUR
T1 - Flow-resistant red blood cell aggregation in morbid obesity
AU - Samocha-Bonet, D.
AU - Ben-Ami, R.
AU - Shapira, I.
AU - Shenkerman, G.
AU - Abu-Abeid, S.
AU - Stern, N.
AU - Mardi, T.
AU - Tulchinski, T.
AU - Deutsch, V.
AU - Yedgar, S.
AU - Barshtein, G.
AU - Berliner, S.
N1 - Funding Information:
Financial support of this work by the Szold Foundation (Hebrew University), the US-Israel Binational Science Foundation, No. 2001203 (to S Yedgar and G Barshtein), the Israel Science Foundation No. 558/03 (to S Yedgar and G Barshtein), the Walter and Greta Stiel Chair for Heart Studies (to S Yedgar), and the Buchmann Joseph Foundation (to D Samocha-Bonet) is acknowledged. We thank Mrs S Levy and Mrs O Fredman for technical assistance. This work was performed in partial fulfillment of the requirements for a PhD degree of Dorit Samocha-Bonet, Sackler faculty of medicine, Tel-Aviv University, Israel.
PY - 2004/12
Y1 - 2004/12
N2 - OBJECTIVE: Enhanced red blood cell (RBC) aggregation has an adverse effect on microcirculatory blood flow and tissue oxygenation. It has been previously shown that obesity is associated with increased RBC aggregation. The objectives of the present study were to further characterize obesity-related RBC aggregation and to examine whether the enhanced aggregation is a plasma- or cellular-dependent process. METHODS: Obese (body mass index (BMI) = 40 ± 6.3 kg/m2, n = 22) and nonobese (BMI = 24 ± 3.4 kg/m 2, n = 18) individuals were evaluated for inflammation markers and aggregation parameters. Aggregation parameters were derived from the distribution of RBC population into aggregate sizes, and from the variation of the distribution as a function of flow-derived shear stress, using a cell flow properties analyzer. To differentiate plasmatic from cellular factors, we determined the aggregation in the presence of autologous plasma or dextran-500kDa and calculated the plasma factor (PF) in the obese group. PF ranges from 0 to 1. When the PF = 1, the aggregation is all due to plasmatic factors, when PF = 0, the altered aggregation depends entirely on cellular factors, whereas 0
AB - OBJECTIVE: Enhanced red blood cell (RBC) aggregation has an adverse effect on microcirculatory blood flow and tissue oxygenation. It has been previously shown that obesity is associated with increased RBC aggregation. The objectives of the present study were to further characterize obesity-related RBC aggregation and to examine whether the enhanced aggregation is a plasma- or cellular-dependent process. METHODS: Obese (body mass index (BMI) = 40 ± 6.3 kg/m2, n = 22) and nonobese (BMI = 24 ± 3.4 kg/m 2, n = 18) individuals were evaluated for inflammation markers and aggregation parameters. Aggregation parameters were derived from the distribution of RBC population into aggregate sizes, and from the variation of the distribution as a function of flow-derived shear stress, using a cell flow properties analyzer. To differentiate plasmatic from cellular factors, we determined the aggregation in the presence of autologous plasma or dextran-500kDa and calculated the plasma factor (PF) in the obese group. PF ranges from 0 to 1. When the PF = 1, the aggregation is all due to plasmatic factors, when PF = 0, the altered aggregation depends entirely on cellular factors, whereas 0
KW - Large aggregate fraction
KW - Plasma factor
KW - Red blood cell aggregation
KW - Shear stress
UR - http://www.scopus.com/inward/record.url?scp=9444283779&partnerID=8YFLogxK
U2 - 10.1038/sj.ijo.0802791
DO - 10.1038/sj.ijo.0802791
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AN - SCOPUS:9444283779
SN - 0307-0565
VL - 28
SP - 1528
EP - 1534
JO - International Journal of Obesity
JF - International Journal of Obesity
IS - 12
ER -
