Flavin Adenine dinucleotide photophysics in ice

Itay Presiado, Dan Huppert*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Steady-state, time-resolved emission and pump-probe techniques were employed to study the nonradiative process of flavin adenine dinucleotide (FAD) in methanol-doped ice and in a deuterated solvent mixture of methanol-d-D 2O in a large range of temperatures of 79-268 K. We found that the nonradiative process depends on the temperature, i.e., the lower the ice temperature the smaller the nonradiative rate. The timeresolved emission is nonexponential, and a large portion of the decay curve could be reasonably fitted to a stretched exponent with α = 0.55 in a large temperature range. We found a relatively large and unexpected isotope effect, KIE = 1.8, on the nonradiative rate. The large nonradiative rate in FAD (compared to flavin mononucleotide (FMN)) possibly arises from a coupled electron-proton transfer from adenine to flavin in a "closed" conformation existing predominantly in an ice crystal.

Original languageEnglish
Pages (from-to)3835-3843
Number of pages9
JournalJournal of Physical Chemistry C
Volume113
Issue number9
DOIs
StatePublished - 5 Mar 2009

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