Fine tuning and efficient T cell activation with stimulatory aCD3 nanoarrays

Jovana Matic, Janosch Deeg, Alexander Scheffold, Itamar Goldstein, Joachim P. Spatz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

Anti-CD3 (aCD3) nanoarrays fabricated by self-assembled nanopatterning combined with site-directed protein immobilization techniques represent a novel T cell stimulatory platform that allows tight control over ligand orientation and surface density. Here, we show that activation of primary human CD4+ T cells, defined by CD69 upregulation, IL-2 production and cell proliferation, correlates with aCD3 density on nanoarrays. Immobilization of aCD3 through nanopatterning had two effects: cell activation was significantly higher on these surfaces than on aCD3-coated plastics and allowed unprecedented fine-tuning of T cell response.

Original languageEnglish
Pages (from-to)5090-5097
Number of pages8
JournalNano Letters
Volume13
Issue number11
DOIs
StatePublished - 13 Nov 2013

Funding

FundersFunder number
Seventh Framework Programme229289

    Keywords

    • Artificial antigen presenting interfaces
    • CD4+ T cells
    • T cell activation
    • anti-CD3 monoclonal antibody
    • block copolymer micellar nanolithography (BCML)
    • nanopattern

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