@article{eb357095d9494d4b8111285b29866f84,
title = "Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: Extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay",
abstract = "Due to the global COVID-19 pandemic, there is a need to screen for novel compounds with antiviral activity against SARS-COV-2. Here we compared chemical composition and the in vitro anti- SARS-COV-2 activity of two different Ulva sp. crude ulvan extracts: one obtained by an HCl-based and another one by ammonium oxalate-based (AOx) extraction protocols. The composition of the crude extracts was analyzed and their antiviral activity was assessed in a cytopathic effect reduction assay using Vero E6 cells. We show that the extraction protocols have a significant impact on the chemical composition, anti- SARS-COV-2 activity, and cytotoxicity of these ulvan extracts. The ulvan extract based on the AOx protocol had a higher average molecular weight, higher charge, and 11.3-fold higher antiviral activity than HCl-based extract. Our results strongly suggest that further bioassay-guided investigation into bioactivity of compounds found in Ulva sp. ulvan extracts could lead to the discovery of novel anti-SARS-CoV-2 antivirals.",
keywords = "Antiviral, Extraction, Macroalgae, SARS-CoV-2, Seaweed, Sulfated polysaccharides, Ulva sp., Ulvan",
author = "Shai Shefer and Arthur Robin and Alexander Chemodanov and Mario Lebendiker and Robert Bostwick and Lynn Rasmussen and Michael Lishner and Michael Gozin and Alexander Golberg",
note = "Publisher Copyright: Copyright 2021 Shefer et al.",
year = "2021",
month = nov,
day = "15",
doi = "10.7717/peerj.12398",
language = "אנגלית",
volume = "9",
journal = "PeerJ",
issn = "2167-8359",
publisher = "PeerJ Inc.",
}
