Fibroblasts adhesion to soy protein films for wound healing: An in vitro study

Dana Gourevich, Lia Ofek, Yael Eichbaum, Lior Wertheim, Ruth Gotlieb, Maya Baranes-Zeevi, Hilla Olami, David Elad, Meital Zilberman

Research output: Contribution to journalArticlepeer-review


Fibroblasts play a significant role in the highly complex process of wound healing. Soy protein isolate (SPI) has recently emerged as an attractive source for biomedical applications, such as being a scaffold for wound dressing. This study was aimed to generate novel SPI based films to serve as wound healing scaffold and to investigate the behaviour of fibroblasts when seeded in the presence of the film and on its surface. We have successfully manufactured several types of SPI film with various thicknesses and evaluated their compatibility as a platform for fibroblastic cells growth. We have then verified that the composition of the film is not toxic to cells and finally grew the cells straight on top of the SPI films. Our early stage studies showed that being a natural polymer, the SPI film absorbs any liquid or dye it comes to contact with and hence causes the cell layers grown on its surface to become indistinguishable. To overcome this issue we have marked the cells prior to their seeding on the SPI with CellTracker™. This has allowed us to visually assess the morphology and growth patterns of the cells on top of the SPI, revealing that although the fibroblasts did not maintain their morphology, they did grow effectively on the film. We were also able to distinguish the lamellipodia which is identified with fibroblasts. The successful visualization of fibroblasts growth on the SPI suggested it could be the next generation of wound dressing in wound healing.
Original languageAmerican English
Pages (from-to)1029-1035
Number of pages7
JournalJSM Burns and Trauma
Issue number1
StatePublished - 2017


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