Abstract
The expression of fibroblast growth factor 10 (FGF-10) has not been studied in human ovarian cortical follicles. The aim of the present study was to investigate the expression of FGF-10 in preantral follicles from fetuses, girls and women. Ovarian samples were obtained from 14 human fetuses at 21-33 gestational weeks and from 35 girls and women aged 5-39 years. The specimens were prepared for detection of the FGF-10 protein by immunohistochemistry. Reverse-transcription PCR was applied to ovarian extracts to identify FGF-10 mRNA transcripts. In fetal tissue, the FGF-10 protein was detected in oocytes in 50% of the samples and in granulosa cells in 30%. In ovarian tissue from girls and women, the FGF-10 protein was detected in oocytes and granulosa cells in all samples. FGF-10 mRNA transcripts were present in all adult and fetal samples tested. The identification of FGF-10 at both the protein and mRNA levels suggests that FGF-10 may contribute to human preantral follicle development.
| Original language | English |
|---|---|
| Pages (from-to) | 396-401 |
| Number of pages | 6 |
| Journal | Reproductive BioMedicine Online |
| Volume | 25 |
| Issue number | 4 |
| DOIs | |
| State | Published - Oct 2012 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
-
SDG 3 Good Health and Well-being
Keywords
- fibroblast growth factor 10 (FGF-10)
- granulosa cells
- human preantral follicles
- immunohistochemistry
- primordial/preantral follicles
- reverse-transcription PCR
Fingerprint
Dive into the research topics of 'Fibroblast growth factor 10 in human ovaries'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver