TY - JOUR
T1 - Fibroblast growth factor 10 in human ovaries
AU - Oron, Galia
AU - Fisch, Benjamin
AU - Zhang, Xiao Yun
AU - Gabbay-Benziv, Rinat
AU - Kessler-Icekson, Gania
AU - Krissi, Haim
AU - Ben-Haroush, Avi
AU - Ao, Asangla
AU - Abir, Ronit
PY - 2012/10
Y1 - 2012/10
N2 - The expression of fibroblast growth factor 10 (FGF-10) has not been studied in human ovarian cortical follicles. The aim of the present study was to investigate the expression of FGF-10 in preantral follicles from fetuses, girls and women. Ovarian samples were obtained from 14 human fetuses at 21-33 gestational weeks and from 35 girls and women aged 5-39 years. The specimens were prepared for detection of the FGF-10 protein by immunohistochemistry. Reverse-transcription PCR was applied to ovarian extracts to identify FGF-10 mRNA transcripts. In fetal tissue, the FGF-10 protein was detected in oocytes in 50% of the samples and in granulosa cells in 30%. In ovarian tissue from girls and women, the FGF-10 protein was detected in oocytes and granulosa cells in all samples. FGF-10 mRNA transcripts were present in all adult and fetal samples tested. The identification of FGF-10 at both the protein and mRNA levels suggests that FGF-10 may contribute to human preantral follicle development.
AB - The expression of fibroblast growth factor 10 (FGF-10) has not been studied in human ovarian cortical follicles. The aim of the present study was to investigate the expression of FGF-10 in preantral follicles from fetuses, girls and women. Ovarian samples were obtained from 14 human fetuses at 21-33 gestational weeks and from 35 girls and women aged 5-39 years. The specimens were prepared for detection of the FGF-10 protein by immunohistochemistry. Reverse-transcription PCR was applied to ovarian extracts to identify FGF-10 mRNA transcripts. In fetal tissue, the FGF-10 protein was detected in oocytes in 50% of the samples and in granulosa cells in 30%. In ovarian tissue from girls and women, the FGF-10 protein was detected in oocytes and granulosa cells in all samples. FGF-10 mRNA transcripts were present in all adult and fetal samples tested. The identification of FGF-10 at both the protein and mRNA levels suggests that FGF-10 may contribute to human preantral follicle development.
KW - fibroblast growth factor 10 (FGF-10)
KW - granulosa cells
KW - human preantral follicles
KW - immunohistochemistry
KW - primordial/preantral follicles
KW - reverse-transcription PCR
UR - http://www.scopus.com/inward/record.url?scp=84867101950&partnerID=8YFLogxK
U2 - 10.1016/j.rbmo.2012.07.002
DO - 10.1016/j.rbmo.2012.07.002
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C2 - 22877940
AN - SCOPUS:84867101950
SN - 1472-6483
VL - 25
SP - 396
EP - 401
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
IS - 4
ER -
