Fibroblast growth factor 10 in human ovaries

Galia Oron, Benjamin Fisch, Xiao Yun Zhang, Rinat Gabbay-Benziv, Gania Kessler-Icekson, Haim Krissi, Avi Ben-Haroush, Asangla Ao, Ronit Abir

Research output: Contribution to journalArticlepeer-review

Abstract

The expression of fibroblast growth factor 10 (FGF-10) has not been studied in human ovarian cortical follicles. The aim of the present study was to investigate the expression of FGF-10 in preantral follicles from fetuses, girls and women. Ovarian samples were obtained from 14 human fetuses at 21-33 gestational weeks and from 35 girls and women aged 5-39 years. The specimens were prepared for detection of the FGF-10 protein by immunohistochemistry. Reverse-transcription PCR was applied to ovarian extracts to identify FGF-10 mRNA transcripts. In fetal tissue, the FGF-10 protein was detected in oocytes in 50% of the samples and in granulosa cells in 30%. In ovarian tissue from girls and women, the FGF-10 protein was detected in oocytes and granulosa cells in all samples. FGF-10 mRNA transcripts were present in all adult and fetal samples tested. The identification of FGF-10 at both the protein and mRNA levels suggests that FGF-10 may contribute to human preantral follicle development.

Original languageEnglish
Pages (from-to)396-401
Number of pages6
JournalReproductive BioMedicine Online
Volume25
Issue number4
DOIs
StatePublished - Oct 2012

Keywords

  • fibroblast growth factor 10 (FGF-10)
  • granulosa cells
  • human preantral follicles
  • immunohistochemistry
  • primordial/preantral follicles
  • reverse-transcription PCR

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