Feverlike temperature is a virulence regulatory cue controlling the motility and host cell entry of typhoidal Salmonella

Dana Elhadad, Michael McClelland, Galia Rahav, Ohad Gal-Mor*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Human infection with typhoidal Salmonella serovars causes a febrile systemic disease, termed enteric fever. Here we establish that in response to a temperature equivalent to fever (39°C-42°C) Salmonella enterica serovars Typhi, Paratyphi A, and Sendai significantly attenuate their motility, epithelial cell invasion, and uptake by macrophages. Under these feverlike conditions, the residual epithelial cell invasion of S. Paratyphi A occurs in a type III secretion system (T3SS) 1-independent manner and results in restrained disruption of epithelium integrity. The impaired motility and invasion are associated with down-regulation of T3SS-1 genes and class II and III (but not I) of the flagella-chemotaxis regulon. In contrast, we demonstrate up-regulation of particular Salmonella pathogenicity island 2 genes (especially spiC) and increased intraepithelial growth in a T3SS-2-dependent manner. These results indicate that elevated physiological temperature is a novel cue controlling virulence phenotypes in typhoidal serovars, which is likely to play a role in the distinct clinical manifestations elicited by typhoidal and nontyphoidal salmonellae.

Original languageEnglish
Pages (from-to)147-156
Number of pages10
JournalJournal of Infectious Diseases
Volume212
Issue number1
DOIs
StatePublished - 1 Jul 2015

Keywords

  • Enteric fever
  • Salmonella
  • flagella
  • invasion.
  • motility
  • paratyphoid
  • pathogenicity
  • pyrexia

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