Fetal cells in the uterine cervix: A source for early non-invasive prenatal diagnosis

Moshe D. Fejgin, Ron Diukman, Yael Cotton, Galina Weinstein, Aliza Amiel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Various non-invasive techniques for prenatal diagnosis have been under investigation. We evaluated the success of fetal sexing using a non-invasive technique for obtaining fetal cells, uterine cervix brushing, in combination with FISH. Thirty pregnant women who completed between 6 and 10 weeks of gestation and who were scheduled to undergo pregnancy termination were included in the study. A Pap smear cytobrush was inserted through the external os to a maximum depth of 2 cm and removed while rotating it a full turn. The material that was caught on the brush was spread on four microscope slides. Two-color FISH was used for fetal sexing. Following pregnancy termination, a placental sample was used for full karyotyping. In 29/30 cases FISH diagnosis was confirmed by chromosomal analysis. The only male case in which a Y chromosome was not seen was from a pregnancy of 6 weeks 6 days gestational age. One case was mosaic of 46,XY/47,XXY (25%). In most cases (7/13) the Y chromosome was already identified in the first analyzed slide. With the use of a cytobrush fetal cells can be easily obtained for the purpose of prenatal diagnosis of chromosomal disorders.

Original languageEnglish
Pages (from-to)619-621
Number of pages3
JournalPrenatal Diagnosis
Issue number8
StatePublished - 2001
Externally publishedYes


  • Cervical brushing
  • FISH
  • Non-invasive prenatal diagnosis


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