@article{b67aabe9b08745269b7f9d9717d7a6a4,
title = "Feed-forward microprocessing and splicing activities at a microRNA-containing intron",
abstract = "The majority of mammalian microRNA (miRNA) genes reside within introns of protein-encoding and non-coding genes, yet the mechanisms coordinating primary transcript processing into both mature miRNA and spliced mRNA are poorly understood. Analysis of melanoma invasion suppressor miR-211 expressed from intron 6 of melastatin revealed that microprocessing of miR-211 promotes splicing of the exon 6-exon 7 junction of melastatin by a mechanism requiring the RNase III activity of Drosha. Additionally, mutations in the 5′ splice site (5′SS), but not in the 3′SS, branch point, or polypyrimidine tract of intron 6 reduced miR-211 biogenesis and Drosha recruitment to intron 6, indicating that 5′SS recognition by the spliceosome promotes microprocessing of miR-211. Globally, knockdown of U1 splicing factors reduced intronic miRNA expression. Our data demonstrate novel mutually-cooperative microprocessing and splicing activities at an intronic miRNA locus and suggest that the initiation of spliceosome assembly may promote microprocessing of intronic miRNAs.",
author = "Janas, {Maja M.} and Mehdi Khaled and Steffen Schubert and Bernstein, {Jacob G.} and David Golan and Veguilla, {Rosa A.} and Fisher, {David E.} and Noam Shomron and Carmit Levy and Novina, {Carl D.}",
year = "2011",
month = oct,
doi = "10.1371/journal.pgen.1002330",
language = "אנגלית",
volume = "7",
journal = "PLoS Genetics",
issn = "1553-7390",
publisher = "Public Library of Science",
number = "10",
}