Feasibility Study - Vitamin D Loading Determination by Ftir-Atr

E. Simana, R. Simian, S. Portnoy, A. Jaffe, B. Z. Dekel

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: The aim of the present study was to develop a simple and accurate way to measure vitamin D levels. Vitamin D nowadays is measured by a variety of methods which their common drawbacks are expensive equipment and the need for high trained technical staff. In this research we measured vitamin D levels by means of Fourier transform infra red method in conjugation with the evanescent wave spectroscopy technique, in order to develop a simpler vitamin D measurement method. Methods: Blood samples were collected from patients with vitamin D deficiency at five intervals before and up to 16 days after they took a dose of 200,000 IU vitamin D3. Samples were measured by the conventional bio-chemical method and by the evanescent wave spectroscopy means. Results: Correlation was found between the vitamin D levels measured by the traditional method and by the evanescent wave spectroscopy technique. The absorption lines occurred prominently in the IR spectral regions of the Amide I (≈1650 cm−1),Amide II (≈1530 cm−1) and the (≈3400 cm−1) absorption band which is attributed to the hydroxyl group indicated by the O-H stretch. In addition, the examination of the blood samples using the evanescent wave spectroscopy with clustering techniques facilitated the discrimination between vitamin D deficiency and normal vitamin D levels. Practical relevance: This study demonstrates the potential of using the Fourier transform infra red method in conjugation with the evanescent wave spectroscopy techniquecoupled with multivariate analysis as a nonexpensive, rapid and accurate alternative to the routine methodologies.
Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalInformatsionno-Upravliaiushchie Sistemy
Volume76
Issue number3
StatePublished - 1 Jun 2015

Keywords

  • PHYSIOLOGICAL effects of vitamin D
  • FOURIER transform infrared spectroscopy
  • VITAMIN D deficiency
  • FTIR-ATR Spectroscopy
  • Mid Infrared
  • Vitamin D

Fingerprint

Dive into the research topics of 'Feasibility Study - Vitamin D Loading Determination by Ftir-Atr'. Together they form a unique fingerprint.

Cite this