TY - JOUR
T1 - Fate Mapping Reveals Origins and Dynamics of Monocytes and Tissue Macrophages under Homeostasis
AU - Yona, Simon
AU - Kim, Ki Wook
AU - Wolf, Yochai
AU - Mildner, Alexander
AU - Varol, Diana
AU - Breker, Michal
AU - Strauss-Ayali, Dalit
AU - Viukov, Sergey
AU - Guilliams, Martin
AU - Misharin, Alexander
AU - Hume, David A.
AU - Perlman, Harris
AU - Malissen, Bernard
AU - Zelzer, Elazar
AU - Jung, Steffen
N1 - Funding Information:
We would like to thank R. Haffner for guidance with the ES cell work and the staff of the Weizmann Animal facility for the excellent care. S.Y. was a recipient of a long-term FEBS fellowship; A.M. was a fellow of the Minerva foundation. This work was supported by the Leir Charitable Foundation, the Wolfson Family Charitable Trust, the Israel Science Foundation (ISF), and the Deutsche Forschungsgemeinschaft (DFG) Research Unit (FOR) 1336. S.J. is a Helmsley Scholar at the Crohn’s & Colitis Foundation.
PY - 2013/1/24
Y1 - 2013/1/24
N2 - Mononuclear phagocytes, including monocytes, macrophages, and dendritic cells, contribute to tissue integrity as well as to innate and adaptive immune defense. Emerging evidence for labor division indicates that manipulation of these cells could bear therapeutic potential. However, specific ontogenies of individual populations and the overall functional organization of this cellular network are not well defined. Here we report a fate-mapping study of the murine monocyte and macrophage compartment taking advantage of constitutive and conditional CX3CR1 promoter-driven Cre recombinase expression. We have demonstrated that major tissue-resident macrophage populations, including liver Kupffer cells and lung alveolar, splenic, and peritoneal macrophages, are established prior to birth and maintain themselves subsequently during adulthood independent of replenishment by blood monocytes. Furthermore, we have established that short-lived Ly6C+ monocytes constitute obligatory steady-state precursors of blood-resident Ly6C- cells and that the abundance of Ly6C+ blood monocytes dynamically controls the circulation lifespan of their progeny.
AB - Mononuclear phagocytes, including monocytes, macrophages, and dendritic cells, contribute to tissue integrity as well as to innate and adaptive immune defense. Emerging evidence for labor division indicates that manipulation of these cells could bear therapeutic potential. However, specific ontogenies of individual populations and the overall functional organization of this cellular network are not well defined. Here we report a fate-mapping study of the murine monocyte and macrophage compartment taking advantage of constitutive and conditional CX3CR1 promoter-driven Cre recombinase expression. We have demonstrated that major tissue-resident macrophage populations, including liver Kupffer cells and lung alveolar, splenic, and peritoneal macrophages, are established prior to birth and maintain themselves subsequently during adulthood independent of replenishment by blood monocytes. Furthermore, we have established that short-lived Ly6C+ monocytes constitute obligatory steady-state precursors of blood-resident Ly6C- cells and that the abundance of Ly6C+ blood monocytes dynamically controls the circulation lifespan of their progeny.
UR - http://www.scopus.com/inward/record.url?scp=84872765982&partnerID=8YFLogxK
U2 - 10.1016/j.immuni.2012.12.001
DO - 10.1016/j.immuni.2012.12.001
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C2 - 23273845
AN - SCOPUS:84872765982
SN - 1074-7613
VL - 38
SP - 79
EP - 91
JO - Immunity
JF - Immunity
IS - 1
ER -
