Fat transport in the locust, Locusta migratoria: The role of protein synthesis

1. 1. When prelabeled fat body was incubated in the presence of hemolymph, diglycerides were released and incorporated into lipoproteins of the hemolymph. 2. 2. When fat body was incubated with ¹⁴C-labeled amino acids, the acids were incorporated into tissue proteins which were subsequently released into the medium. Protein release was not dependent on the presence of hemolymph. 3. 3. Gel electrophoresis of the released protein showed that the distribution of radioactivity along the gel coincided with the protein bands of the hemolymph. 4. 4. Addition of cycloheximide inhibited the incorporation of amino acids into proteins; the release of preformed proteins was not affected. 5. 5. Cycloheximide did not inhibit the incorporation of ¹⁴C-labeled palmitate into glycerides, neither was the release of glycerides from the "inhibited" tissue affected.