F20C, a new fluorescent membrane probe, moves more slowly in malignant and mitogen-transformed cell membranes than in normal cell membranes

Nechama S. Kosower*, Edward M. Kosower, Shlomo Lustig, Dov H. Pluznik

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

New fluorescent probes of membrane mobility can be introduced into cell membranes at single points with particles of a membrane mobility agent, A2C. The initial entry of fluorescence from the particle into the cell membrane and the subsequent lateral spread of fluorescence have been observed for cells in suspension. A dramatic difference between the behavior of normal lymphocytes and that of mitogen-transformed and mastocytoma cells is found. Both the initial entry and the spreading of fluorescence are much slower in the transformed and tumor cells than in the normal cells at 18°C. Entry and spread of fluorescence in normal cells become slow enough to be observed only at 12°C or below.

Original languageEnglish
Pages (from-to)128-136
Number of pages9
JournalBBA - Biomembranes
Volume507
Issue number1
DOIs
StatePublished - 2 Feb 1978

Funding

FundersFunder number
Israeli Cancer Society
Research Authority of Bar-Ilan University

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