Extracellular matrix vesicles in rat bone after parathyroidectomy

J. Sela, I. Bab, A. Muhlrad, U. A. Liberman, D. Deutsch

Research output: Contribution to journalArticlepeer-review

Abstract

The enzymatic activity of bone matrix vesicles from parathyroidectomized rats was determined and compared to the activity of vesicles from sham operated and normal animals. The vesicles were isolated from the alveolar bone by collagenase digestion and differential centrifugation and further purified on a discontinuous sucrose density gradient. The amount of extractable protein and the activity of alkaline phosphatase, acid phosphatase, and ATPase in the vesicle fractions thus obtained did not differ significantly from the values characteristic of preparations from control rats. It may therefore be suggested that parathyroid hormone depletion and the associated hypocalcemia have no significant effect on the occurrence and phosphatase activity of bone matrix vesicles.

Original languageEnglish
Pages (from-to)451-457
Number of pages7
JournalCell and Tissue Research
Volume228
Issue number3
DOIs
StatePublished - Jan 1983
Externally publishedYes

Keywords

  • Bone matrix vesicles
  • Mineralization
  • Parathyroid hormone
  • Rat

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