Extending the Host Range of Bacteriophage Particles for DNA Transduction

Ido Yosef, Moran G. Goren, Rea Globus, Shahar Molshanski-Mor, Udi Qimron*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


A major limitation in using bacteriophage-based applications is their narrow host range. Approaches for extending the host range have focused primarily on lytic phages in hosts supporting their propagation rather than approaches for extending the ability of DNA transduction into phage-restrictive hosts. To extend the host range of T7 phage for DNA transduction, we have designed hybrid particles displaying various phage tail/tail fiber proteins. These modular particles were programmed to package and transduce DNA into hosts that restrict T7 phage propagation. We have also developed an innovative generalizable platform that considerably enhances DNA transfer into new hosts by artificially selecting tails that efficiently transduce DNA. In addition, we have demonstrated that the hybrid particles transduce desired DNA into desired hosts. This study thus critically extends and improves the ability of the particles to transduce DNA into novel phage-restrictive hosts, providing a platform for myriad applications that require this ability.

Original languageEnglish
Pages (from-to)721-728.e3
JournalMolecular Cell
Issue number5
StatePublished - 1 Jun 2017


FundersFunder number
European Research Council336079
Israel Science Foundation268/14
Tel Aviv University
Ministry of Health, State of Israel9988-3


    • Escherichia coli
    • T7 bacteriophage
    • host recognition
    • hybrid particle
    • transducing particles


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